响应面法优化香菇柄中多酚提取工艺
目的 探究超声耦合双水相法提取香菇柄中多酚类物质的最佳工艺。方法 采用超声耦合双水相法对香菇柄中多酚进行提取, 探讨硫酸铵质量分数、液料比、超声时间、超声功率对多酚含量的影响, 在单因素试验的基础上, 设计响应面优化试验, 确定最佳提取工艺。结果 各因素对多酚含量的影响由大到小依次为: 超声功率>硫酸铵质量分数>液料比>超声时间。超声耦合双水相法提取香菇柄中多酚的最佳工艺为: 超声功率125 W, 硫酸铵质量分数40 g/100 mL, 液料比30:1 (mL/g), 超声时间40 min。在此条件下, 提取的多酚含量预测值为31.746 mg/g, 试验值为(31.746±0.008) mg/g, 试验值与预测值基本相符。结论 超声耦合双水相法提取条件温和、操作简单, 与传统乙醇浸提法相比, 不仅节约提取时间, 还能提高多酚含量达60%, 适于香菇柄中多酚类物质的提取研究。
Objective To optimize ultrasound combined aqueous two-phase extraction of polyphenols from Lentinus edodes stems. Methods Polyphenols in Lentinus edodes stems were extracted by ultrasound combined aqueous two-phase extraction. The effects of operating conditions such as ammonium sulfate mass fraction, liquid-solid ratio, ultrasonic time and ultrasonic power were investigated. Four-factor three-level experiments based on response surface analysis method were applied to optimize the extraction conditions. Results The extraction yield of polyphenols was affected by various factors in a descending order as: ultrasonic power, ammonium sulfate mass fraction, liquid-solid ratio and ultrasonic time. The optimum extraction conditions were as follows: ultrasonic power of 125 W, liquid-solid ratio of 30:1 (mL/g), ultrasonic time of 40 min, ammonium sulfate mass fraction of 40 g/100 mL. Under these conditions, the prediction value of extraction yield of polyphenols was 31.746 mg/g, and the experimental value was (31.746±0.008) mg/g, which was almost consistent with the prediction value. Conclusion The extraction condition of the ultrasound combined aqueous two-phase extraction method is moderate and the operation is simple. Compared with traditional ethanol extraction method, not only the extraction time of aqueous two-phase extraction was saved obviously, but the yield of polyphenols in Lentinus edodes stems was increased by 60%, which is suitable for the extraction study of polyphenols in Lentinus edodes stems.
标题：响应面法优化香菇柄中多酚提取工艺
英文标题：Optimization of extraction process for polyphenol from Lentinus edodes stem by response surface methodology
作者：
缪彬彬 吉林大学食品科学与工程学院
徐艳阳 吉林大学食品科学与工程学院
王一迪 吉林大学食品科学与工程学院
关欢欢 吉林大学食品科学与工程学院
张贵阳 吉林大学食品科学与工程学院
李欣欣 吉林大学食品科学与工程学院
中文关键词:香菇柄,多酚,超声耦合双水相提取,响应面法,
英文关键词:Lentinus edodes stem,polyphenol,ultrasound combined aqueous two-phase extraction,response surface analysis,
发表日期：2016-03-19
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- 2.57 MB
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