大球盖菇蛋白提取及抗氧化性研究
目的 明确大球盖菇蛋白的提取工艺及体外抗氧化特性。方法 将新鲜大球盖菇烘干, 在溶剂选择、料液比、pH、浸提温度及浸提时间单因素试验基础上采用Box-Behnken实验设计对碱溶酸沉大球盖菇蛋白工艺进行优化, 并以维生素C(VC)作为阳性对照进行体外抗氧化测定。结果 大球盖菇蛋白最佳提取工艺为: 以水为提取剂、pH 12、料液比1:30 g/mL、浸提时间60 min、浸提温度45 ℃, 在此条件下蛋白提取率预测值为37.87%, 实际验证蛋白提取率为37.54%±0.24%, 二者差异不显著。大球盖菇蛋白等电点为pH 4.2。大球盖菇蛋白有一定的还原力, 对1,1-二苯基-2-三硝基苯肼(DPPH)自由基、羟自由基(?OH)有一定的清除能力, 清除自由基的半抑制浓度(IC50)分别为2.683 mg/mL、6.094 mg/mL。结论 碱法是一种简单有效的大球盖菇蛋白提取方法, 提取的蛋白有一定的抗氧化特性。
Objective To clarify the extraction process of Stropharia rugosoannulata protein and measure its antioxidant activity. Methods The preparation of protein from dried fruit bodies by alkali extraction and acid precipitation and Box-Behnken experiment design was used in combination with single-factor experiments to obtain the optimal extraction conditions. In vitro antioxidant assay was performed with vitamin C (VC) as positive control. Results The optimal extraction conditions as follows: solvent was water, extraction pH was 12, the material-liquid ratio was 1:30 (g/mL), extraction time was 60 min, and extraction temperature was 45 ℃. Under the conditions, the theoretical predicted extraction yield of protein was 37.87%, and the verification value was 37.54%±0.24% in the repeated trials, which was no significant between them. The isoelectric point of Stropharia rugosoannulata protein was measured to be pH 4.2. Stropharia rugosoannulata protein showed certain reducing power, and certain scavenging activity against 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical and hydroxyl radicals, the semi-inhibitory concentration (IC50) were 2.683 mg/mL and 6.094 mg/mL, respectively. Conclusion Alkali extraction is an effective protein extraction method from Stropharia rugosoannulata, and the extracted protein has certain antioxidant activity.
标题：大球盖菇蛋白提取及抗氧化性研究
英文标题：Extraction and antioxidant activity of Stropharia rugosoannulata protein
作者：
崔晓瑞 福建农林大学食品科学学院
王丽 北京农业职业学院
石菲菲 福建农林大学食品科学学院
潘妍 北京农业职业学院
贾红亮 北京农业职业学院
刘小飞 北京农业职业学院
李淑荣 北京农业职业学院
宋洪波 福建农林大学食品科学学院
中文关键词:大球盖菇,蛋白,响应面法,抗氧化能力,
英文关键词:Stropharia rugosoannulata,protein,response surface methodology,antioxidant activity,
发表日期：2018-09-19
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