不同类型瓶装水中铜绿假单胞菌的数量变化研究
目的 探究室温储存条件下, 不同类型瓶装饮用水中铜绿假单胞菌的数量变化趋势。方法 将计数好的铜绿假单胞菌菌悬液分别加入到纯净水、矿物质水、天然深层湖水、天然矿泉水等不同类型的瓶装饮用水中, 跟踪检测保存不同天数后, 水中的菌浓度变化情况。结果 纯净水中铜绿假单胞菌的活菌数量在保存期间一直衰减, 且菌的初始浓度低于2.5×105 CFU/250 mL时, 在保存90 d后未检出活菌。矿物质水、天然深层湖水、天然矿泉水中, 虽然铜绿假单胞菌的初始添加数量不一样, 但均先出现一次明显的增长, 然后再迅速下降, 之后虽偶有小幅回升的情况, 但总体呈衰减趋势; 保存90 d后, 数量分别为50、300、2000 CFU/mL。结论 铜绿假单胞菌的数量在纯净水中直接衰减; 在含有矿物元素的饮用水中, 菌的数量呈现先增后减的趋势, 并在3个月后依然保持较高的菌浓度, 且活菌数量随着矿物元素的种类及浓度的增加而提升。
Objective To explore the change trend of Pseudomonas aeruginosa in different types of drinking water under room temperature storage. Methods The counted P. aeruginosa suspension was added to different types of drinking water, such as pure water, mineralized water, natural deep lake water and mineral water, then the change of bacteria concentration in water was detected continuously after different days. Results The number of P. aeruginosa in purified water declined during storage. When the initial concentration was less than 2.5×105 CFU/250 mL, no viable bacteria was detected after 90 days of storage. Although the initial amount of P. aeruginosa added in mineralized water, natural deep lake water and mineral water was different, there was a significant increase first and then a rapid falling. Despite there were occasional rebounds, it showed a general trend of attenuation. After 90 days of storage, the number of P. aeruginosa was 50, 300 and 2000 CFU/mL, respectively. Conclusion The number of P. aeruginosa declines directly in pure water. While the number of bacteria firstly increases and then decreases, and still remains a high concentration after 3 months in drinking water containing mineral elements. Furthermore, the number of living bacteria increases with the increase of types and concentrations of mineral elements.
标题：不同类型瓶装水中铜绿假单胞菌的数量变化研究
英文标题：Study on the quantity variation of Pseudomonas aeruginosa in different types of bottled water
作者：
潘波 杭州娃哈哈集团有限公司质量监控部
曾志明 杭州娃哈哈集团有限公司质量监控部
沈中锋 杭州娃哈哈集团有限公司质量监控部
毛勇 杭州娃哈哈集团有限公司质量监控部
吴平 杭州娃哈哈集团有限公司质量监控部
中文关键词:铜绿假单胞菌,包装饮用水,矿物元素,菌浓度,
英文关键词:Pseudomonas aeruginosa,drinking water,mineral elements,bacterial concentration,
发表日期：2018-12-23
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