动植物油脂中丙二醛测定方法的优化
目的 对GB/T 5009.181-2003和GB/T 8937-2006中食用动植物油脂中丙二醛的测定方法进行优化。方法 样品采用三氯乙酸混合溶液经50 ℃恒温水浴震荡提取, 与硫代巴比妥酸(thiobarbituric acid, TBA)作用生成粉红色化合物, 在532 nm处采用分光光度计法测定。结果 丙二醛在0~2.5 mg/L范围内线性关系良好, r=0.9995, 方法检出限为0.1 mg/kg, 回收率在88.0%~100.0%之间, 日内精密度和日间精密度小于6.09 %。结论 该方法扩大了检测范围, 操作简便准确, 灵敏度高, 可适用于动植物油脂中丙二醛的测定。
Objective To optimize the method of GB/T 5009.181-2003 and GB/T 8937-2006 fordetermination of propyldialdehyde in animal and vegetable oils. Methods Samples were extracted with trichloroacetic acid solvent at 50 ℃ by constant temperature water bath oscillators, the filtered solution was derivated by thiobarbituric acid for spectrophotometric test at a wavelength of 532 nm. Results Propyldialdehyde had a good linearity in the range of 0~2.5 mg/L with the correlation coefficient of 0.9995. The limit of detection was 0.1 mg/kg, and the recovery was in the range of 88.0%~100.0% with inter-day precision and within-day precision less than 6.09%. Conclusion The improved method expands the scope of tests, which is more simple, rapid, accurate, and suitable for the determination of propyldialdehyde in animal and vegetable oils.
标题：动植物油脂中丙二醛测定方法的优化
英文标题：Amendment of method for determination of propyldialdehyde in animal and vegetable oils
作者：
赵永锋 广西出入境检验检疫局检验检疫技术中心
吴玉杰 广西出入境检验检疫局检验检疫技术中心
吕春秋 广西出入境检验检疫局检验检疫技术中心
欧玉玲 广西出入境检验检疫局检验检疫技术中心
中文关键词:动物油脂,植物油脂,丙二醛,测定方法,优化,
英文关键词:animal oil,vegetable oils,propyldialdehyde,determination method,optimization,
发表日期：2016-06-15
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