Objective To establish a method for detecting avian influenza virus of H5, H7 and H9 subtype using surface plasmon resonance system. Methods The hemagglutinin protein of H5, H7 and H9 subtype which expressed by prokaryotic was coupled on the CM5 chip. The reference antigen of 3 subtypes was diluted gradiently using the HBS buffer and reacted with fixed concentration of 3 subtypes of AIV antiserum respectively. With the preparation of standard curve using competing test, we could calculate the concentration of the sample and detection sensitivity limit. Then the antibody of different subtypes and 5 other poultry disease viruses were tested specificity. Results Various subtypes had no cross reaction between each other, and no positive response of the signals between other poultry virus based on the surface plasmon resonance method. The limit of HA recombinant proteins detection could be reached 0.5 μg/mL. Conclusion The study showed that the method could be used for identifying major epidemic subtypes of avian influenza virus synchronously.
标题：利用表面离子共振系统检测三种禽流感病毒 亚型的研究
英文标题：Application of the surface plasmon resonance system for identifying subtype of avian influenza A virus
