Objective To determine the residue of nitrofuran metabolites inglucosamine chondroitin tablets by liquid chromatography tandem mass spectrometry. Methods The sample was derived 16 h in acid condition (pH<2), and then extracted and concentrated by ethyl acetate. The extract was dissolved and filtered by methanol-water and determined by liquid chromatography tandem mass spectrometry. The isotope internal standard method was used in the experiment to compensate the loss in the pretreatment. Results The recovery experiments were conducted at levels of 0.5, 1.0 and 2.0 μg/kg, and the recoveries of the four metabolites ranged in 82.7%-106.7% with RSD≤12.3%. The limit of detection was 0.5 μg/kg. Conclusion The method has good accuracy, sensitivity and stability and can be used for the determination of residual nitrofuran metabolites in glucosamine chondroitin tablets.
标题：液相色谱串联质谱法测定氨糖软骨素片中硝基呋喃类代谢物残留
英文标题：Determination of nitrofuran metabolites residues in glucosamine chondroitin tablets by liquid chromatography tandem mass spectrometry
