液相色谱法快速检测染色梅鱼中日落黄、柠檬黄的含量
目的 建立一种快速、低成本检测染色梅鱼中日落黄、柠檬黄的方法。方法 将染色梅鱼剥皮, 鱼皮经70%甲醇氨水提取液提取, 经Eclipse XDB-C18色谱柱分离, 以甲醇和乙酸铵水溶液为流动相, 进行梯度洗脱, 流速为1.0 mL/min, 柱温35 ℃, 利用紫外检测器检测日落黄、柠檬黄的含量。结果 日落黄、柠檬黄在1.25、2.5、12.5、25 mg/kg添加水平的加标回收率为80%~90%, 相对标准偏差小于10%(n=6), 柠檬黄检出限为0.05 mg/kg, 日落黄检出限为0.025 mg/kg。结论 该方法快速、准确、成本低, 适合染色梅鱼中日落黄、柠檬黄的快速检测。
Objective To develop a method for determination the sunset yellow and lemon yellow in Collichthys niveatus by liquid chromatography. Methods The dyed Collichthys niveatus was peeled, and the fish skin was extracted with 70% methanol ammonia water extract. The samples were separated with a Eclipse XDB-C18 column at the flow rate of 1.0 mL/min by gradient elution using methanol and ammonium acetate in aqueous solution as the mobile phase, the temperature of column was 35 ℃, and the content of sunset yellow and lemon yellow was detected by ultraviolet detector. Results The standard recoveries of sunset yellow and lemon yellow at 1.25, 2.5, 12.5, 25 mg/kg were 80%~90%, and the relative standard deviations were less than 10%(n=6). The detection limit of lemon yellow was 0.05mg/kg, and the detection limit of sunset yellow was 0.025mg/kg. Conclusion This method is rapid, accurate and low cost, which is suitable for the rapid detection of sunset yellow and lemon yellow in the dyed Collichthys niveatus.
标题：液相色谱法快速检测染色梅鱼中日落黄、柠檬黄的含量
英文标题：Rapid detection of sunset yellow and lemon yellow in Collichthys niveatus by liquid chromatography
作者：
严卓彦 舟山市普陀区公共检验检测创新服务中心
吴俣 舟山市普陀区公共检验检测创新服务中心
中文关键词:日落黄,柠檬黄,梅鱼,液相色谱法,
英文关键词:sunset yellow,lemon yellow,Collichthys niveatus,liquid chromatography,
发表日期：2018-09-29
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