高效液相色谱法测定大豆磷脂类保健食品中磷脂酰胆碱含量
目的 建立高效液相色谱法(high performance liquid chromatography, HPLC)测定大豆磷脂类保健食品中磷脂酰胆碱含量的分析方法。方法 采用高效液相色谱仪、Kromasil 100-5-SIL硅胶柱, 以流动相正己烷:异丙醇:水=0.25:4:1(V:V:V), 流速为0.5 mL/min, 检测波长为207 nm, 柱温为30 ℃, 进样量10 μL, 进行检测。结果 磷脂酰胆碱浓度为0.1~2.0 mg/mL时, 线性关系良好, 回归方程的相关系数r2=0.9998, 相对标准偏差(relative, standard deviation, RSD)为0.50%, 平均回收率为98.01%~98.73%。结论 该方法高效, 准确, 简便, 适合于检测大豆磷脂类保健食品中磷脂酰胆碱的含量。
Objective To establish a method for the determination of phosphatidylcholine from soybean phospholipid health products by high performance liquid chromatography (HPLC). Methods The sample was tested by HPLC with Kromasil 100-5-SIL silica gel column. Mobile phase was n-hexane: isopropanol: water=0.25:4:1 (V:V:V) with flow rate of 0.5 mL/min, the detection wavelength was 207 nm, column temperature was 30 ℃, and injection volume was 10 μL. Results When the concentration of phosphatidylcholine was 0.1-2.0 mg/mL, the linear relationship was good. The correlation coefficient of regression equation was r2=0.9998, the relative standard deviation (RSD) was 0.50%, and the average recoveries were 98.01%-98.73%. Conclusion This method is efficient, accurate and simple, which is suitable for detecting the content of phosphatidylcholine in soybean phospholipid health food.
标题：高效液相色谱法测定大豆磷脂类保健食品中磷脂酰胆碱含量
英文标题：Determination of phosphatidylcholine from soybean phospholipid health products by high performance liquid chromatography
作者：
刘彬丽 中国食品药品检定研究院
唐亚利 中国食品药品检定研究院
金绍明 中国食品药品检定研究院
宁霄 中国食品药品检定研究院
曹进 中国食品药品检定研究院
中文关键词:大豆磷脂,磷脂酰胆碱,保健食品,高效液相色谱法,
英文关键词:soybean phospholipids,phosphatidylcholine,health food,high-performance liquid chromatography method,
发表日期：2018-10-17
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