液相色谱法测定乳粉中乳糖方法优化
目的 研究并优化GB 5413.5-2010《食品安全国家标准 婴幼儿食品和乳品中乳糖、蔗糖的测定》样品制备步骤, 解决婴幼儿配方乳粉中乳糖检测结果偏低的现象, 保证乳糖检测的准确性。方法 在样品溶液制备过程中, 采取30 mL水溶解乳粉, 并向容量瓶中加入1 mL 3%的乙酸水溶液协助蛋白沉淀, 进而降低乙腈在样品溶液中的利用比例, 保证样品制备过程中乳糖的充分溶解, 最终通过液相色谱测定。结果 同一份样品经不同的样品制备方式, 于相同的色谱条件下检测, 依据本法制得样品测定结果比遵循国标条件制得样品的结果高出7%, 并且结果平行性良好。结论 通过方法学研究, 证实该方法具有适用性、重复性、准确度以及线性范围良好的特点, 并且操作简便, 适用于婴幼儿配方乳粉中乳糖的检测。
Objective To investigate and optimize sample preparation process of GB 5413.5-2010 “National food safety standard Determination of lactose and sucrose in foods for infants and young children, milk and milk products ”, so as to solve the problem of low lactose detection result in infant formula powder, and guarantee the accuracy of detection for lactose in milk powder. Methods During the preparation of the sample solution, 30 mL water was used to dissolve the milk powder, and 1 mL 3% acetic acid solution was added to the volumetric flask to assist protein precipitation. Thus, the proportion of acetonitrile in the sample solution was reduced, and the full dissolution of lactose in the sample preparation process was guaranteed. Samples were finally detected by liquid chromatography. Results The results of the same sample detected in the same chromatographic conditions were different by different sample preparation methods. The result detected by the established method was 7% higher than that of the sample prepared using the national standard conditions, and the results had good parallelism. Conclusion The methodology study confirm that the established method has good applicability, repeatability, accuracy, linearity range, and simple operation, which is suitable for the detection of lactose in infant formula powder.
标题：液相色谱法测定乳粉中乳糖方法优化
英文标题：Optimization of determination method of lactose in milk powder by liquid chromatography
作者：
高山 雅培(嘉兴)营养品有限公司检测中心
程敏 雅培(嘉兴)营养品有限公司检测中心
张洪元 雅培(嘉兴)营养品有限公司检测中心
谢玄 雅培(嘉兴)营养品有限公司检测中心
中文关键词:乳糖,乳粉,液相色谱法,
英文关键词:lactose,milk powder,liquid chromatography,
发表日期：2016-12-28
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