[生物] 菌落总数能力验证样品均匀性和稳定性的研究

202 0
2025-1-30 09:30 | 查看全部 阅读模式

菌落总数能力验证样品均匀性和稳定性的研究
目的  研究制备适用于菌落总数能力验证冷冻干燥样品的方法, 并对样品的稳定性和均匀性进行评估。方法  采用真空冷冻干燥方式分别制备菌落总数能力验证阳性样品与阴性样品, 西林瓶真空包装、4 ℃冷藏贮存, 制备出以脱脂奶粉为基质的均匀性好、稳定性强的菌落总数能力验证样品。通过随机抽样以评估样品的均匀性; 通过观察样品在12 w内不同温度下菌落总数的测试结果以评估样品的稳定性。结果  随机抽检的样品具有较好的均匀性; 贮存温度对样品稳定性有较大影响, 高温明显降低样品稳定性; 通过保存温度和时间的稳定性检验显示测试结果符合预期效果, 样品具有较好的稳定性。结论  建立了有效的菌落总数能力验证样品制备的方法与评价程序。

Objective  The preparation of lyophilized samples were applied in bacterial count proficiency testing, to assess the homogeneity and stability of samples.Method  Bacterial count proficiency testing positive samples and negative samples were made by vacuum freeze drying method in penicillin bottles vacuum packaging and stored at 4 ℃. The uniformity of the proficiency testing samples was evaluated by random sampling, and the stability of proficiency testing samples was kept for 12 weeks. Results  Random samples had a preferable accordance ratio, and could keep stable for at least 12 weeks while stored at different temperatures. Suitable bacterial count proficiency testing samples and effective sample preparation and evaluation process were established. Conclusion  Effective bacterial count proficiency testing sample preparation method and evaluation process were established

标题:菌落总数能力验证样品均匀性和稳定性的研究
英文标题:Homogeneity and stability of samples used by bacterial count

作者:
刘芳 深圳市计量质量检测研究院
杨平 深圳市计量质量检测研究院
李传礼 深圳市计量质量检测研究院

中文关键词:菌落总数,能力验证,均匀性,稳定性,
英文关键词:Proficiency testing,Bacterial count,Homogeneity,Stability,

发表日期:2015-10-13
文件大小:
681.05 KB
高速下载
2026 资料下载 联系邮件:1991591830#qq.com 浙ICP备2024084428号-1