高压液相色谱法测定水产品中呋喃苯烯酸钠残留

高压液相色谱法测定水产品中呋喃苯烯酸钠残留
目的 建立高效液相色谱法测定水产品中呋喃苯烯酸钠残留量的分析方法。方法  水产品样品经0.5%甲酸乙腈（v/v）提取后，氨水、正己烷液-液萃取去除杂质，Zorbax SB-C18为分析柱，0.1%甲酸∶乙腈（58:42，v/v）为流动相，经DAD检测器（λ=398 nm）测定，基质标准溶液外标法定量。结果 方法的线性范围为50～1000 μg/L，相关系数r＞0.998，检出限5 μg/kg，定量限10 μg/kg，在10 ~ 100 μg/kg添加水平下平均回收率为87.1% ~ 102%，相对标准偏差（relative standard deviation，RSD）为4.3% ~ 8.4%。结论 该方法能满足水产品中呋喃苯烯酸钠残留量测定的需要。

Objective  To establish an analytical method for the determination of sodium nifurstylenate in aquatic products by high performance liquid chromatography method. Method  Samples were extracted by 0.5% formic acid-acetonitrile（v/v）, followed by clean-up by liquid-liquid extraction with ammonium hydroxide and n-hexane. A Zorbax SB-C18 column was adopted with the mobile phase of 0.1% formic acid- acetonitrile（58:42，v/v）. Sodium nifurstylenate was detected by DAD detector at 398 nm, and quantitively analysed by external standard. Results  The method showed a good linearity in the range of 50-1000 μg/L with the correlation coefficients over 0.998. The limit of detection（LOD） was 5 μg/kg, and the limit of quantitation(LOQ)  was 10 μg/kg. The average recoveries were 87.1%-102% at the spiked level of 10-100 μg/kg with the relative standard deviation (RSD) of 4.3%-8.4%. Conclusion  This method can meet the requirement of fast and accurate determination of sodium nifurstylenate in aquatic products.

标题：高压液相色谱法测定水产品中呋喃苯烯酸钠残留
英文标题：Determination of residues of sodium nifurstylenate in aquatic products by high pressure liquid chromatography

作者：
徐英江 山东省海洋资源与环境研究院,山东省海洋生态修复重点实验室
任传博 烟台山水海产有限公司
宫向红 山东省海洋资源与环境研究院,山东省海洋生态修复重点实验室
李佳蔚 烟台山水海产有限公司
黄会 烟台山水海产有限公司

中文关键词:呋喃苯烯酸钠,水产品,高压液相色谱法,
英文关键词:sodium nifurstylenate,aquatic products,high pressure liquid chromatography,

发表日期：2013-10-30

高压液相色谱法测定水产品中呋喃苯烯酸钠残留.pdf

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