气相色谱高分辨飞行时间质谱法快速筛查水果中283种农药残留
目的 建立气相色谱高分辨飞行时间质谱法(gas chromatography time-of-flight mass spectrometry, GC-TOF/MS)快速筛查水果中283种农药残留量的分析方法。方法 利用气相色谱高分辨飞行时间质谱结合MassHunter、PCDL软件建立283种农药的EI精确质量谱库, 试样经QuEChERS方法进行萃取净化后, 采用EI全扫描监测, 通过PCDL谱库全离子筛查模式检索, 以保留时间、特征离子精确质量数、分子离子同位素信息等作为定性依据。由谱库选择质量数较大、丰度比较高的精确质量离子为定量离子, 进行定量测定。 结果 在空白苹果、葡萄、橙3种水果中添加10、20、40 μg/kg 3个浓度水平下, 方法的平均回收率65.9%~129.1%, 相对标准偏差(relative standard deviation, RSD)2.8%~11.6%, 方法的最低检出浓度可达0.5~ 10 μg/kg。结论 该方法简便、快速、灵敏、精密度好, 是水果中常见农药残留有效快速筛查技术。
Objective To establish a method for the rapid simultaneous screening of 283 pesticide residues in the fruits by gas chromatography time-of-flight mass spectrometry(GC-TOF/MS). Methods A total of 283 pesticides were injected to GC-TOF/MS to create the personal compound database, which exact mass of ions from the first-stage mass spectrum, using MassHunter and PCDL software. The fruit sample was extracted and purified using QuEChERS approach. Extracts were analyzed by GC-TOF/MS operating in high resolution TOF mode with electron ionization. The qualitative screening and quantitative analysis fulfilled by full scan mode, based on an accurate-mass database, which included the retention time, characteristic ion precise mass and molecular ion isotope. Results The three different concentrations levels of 10, 20 and 40 μg/kg were spiked to apple, grape, orange samples. The average recoveries ranged from 65.9% to 129.1%, with relative standards deviation (RSD) of 2.8%~11.6%. The limits of detection were in the range of 0.5~10 μg/kg for the pesticides. Conclusion The experimental results show that the multi-residue analytical method is simple, rapid, sensitive and accurate, it may be effectively applied to the monitoring of the common pesticides in the fruit samples.
标题：气相色谱高分辨飞行时间质谱法快速筛查水果中283种农药残留
英文标题：Rapid screening of 283 pesticide residues in fruits by gas chromatography time-of-flight mass spectrometry
作者：
吴洁珊 拱北海关
倪清泉 珠海生产力促进中心
任永霞 拱北海关
黄晶 拱北海关
梁宁 拱北海关
冯爽 安捷伦科技(中国)有限公司广州分公司
中文关键词:气相色谱高分辨飞行时间质谱法,农药,多残留,水果,
英文关键词:gas chromatography high resolution time-of-flight mass spectrometry,pesticide,multi-residue,fruit,
发表日期：2020-01-10
- 文件大小:
- 2.44 MB
- 下载次数:
- 60
-
高速下载
|
|
