Objective To study the effect of fluorescent brightener on micronucleus and gene mutation of Chinese hamster ovary cells (CHO), and to discuss the mechanism of genetic damage of mammalian cell in vitro. Methods The genetic damage of CHO cells treated with different concentrations of fluorescent brightener 28 was detected with the method of mammalian cell micronucleus test and mammalian cell gene mutation test in vitro. Results There was no significant difference of hprt gene mutation frequencies and micronucleus rates between negative control group and 0.5556 mg/mL and 1.6667 mg/mL fluorescent brightener 28 treated groups. The hprt gene mutation frequency and micronucleus rate of CHO cells with 5.000 mg/mL fluorescent brightener 28 were significant higher than that of negative control group (P<0.05). The hprt gene mutation frequency and micronucleus rate of CHO cells in positive control group were significant higher than that of negative control group (P<0.01). Conclusion Fluorescent brightener 28 may induce genetic damage in cultured mammalian cells.
标题：荧光增白剂致中国仓鼠卵巢细胞微核形成与 基因突变研究
英文标题：Effect of fluorescent brightener on micronucleus and gene mutation of Chinese hamster ovary cells
