新型QuEChERS方法结合液相色谱串联质谱法快速测定猪肝中-受体激动剂残留
目的 建立一种基于新型QuEChERS材料测定猪肝中?-受体激动剂的方法。方法 猪肝样品经酶解、酸化乙腈提取后, 提取液用增强基质去脂质材料(EMR-lipid)脱脂, 盐析浓缩后经液相色谱质谱联用仪测定, 稳定同位素内标法定量。结果 19种?-受体激动剂在0.5~10.0 ?g/kg范围内线性关系良好, 高、中、低浓度日内、日间精密度小于20%, 回收率在60%~120%之间。结论 本方法简便快速, 适宜于大批量样品的快速筛选和定量分析。
Objective To develop a method to determine the ?-agonists residuals in pig liver based on a novel QuEChERS material. Methods The sample of pig liver was enzymatic hydrolysis and extracted by aci-dified acetonitrile. The extraction was cleaned-up by enhanced matrix removal (EMR-lipid) material. After polished and salted out, the analytes were detected by liquid chromatography-tandem mass spectrometry (LC-MS/MS). Stable isotopes were used as internal standard. Results Nineteen ?-agonists were detected at the range between 0.5 ?g/kg and 10.0 ?g/kg with good linearity. The intra- and inter-precision were lower than 20%, and the recoveries were between 60% and 120% with high, medium and low concentrations. Conclusions The method is simple, rapid and effective, and it is suitable for rapid screening and quantitative analysis of large scale samples.
标题：新型QuEChERS方法结合液相色谱串联质谱法快速测定猪肝中-受体激动剂残留
英文标题：Rapid determination of -agonists residuals in pig liver by liquid chromatography- tandem mass spectrometry combined with novel QuEChERS method
作者：
曲斌 江苏省畜产品质量检验测试中心
耿士伟 江苏省畜产品质量检验测试中心
陆桂萍 江苏省畜产品质量检验测试中心
蒋天梅 江苏省畜产品质量检验测试中心
邵德佳 江苏省畜产品质量检验测试中心
陈蓉 中国药科大学理学院
郭良雪 安捷伦科技(中国)有限公司
中文关键词:-受体激动剂,残留,QuEChERS,液相色谱串联质谱法,
英文关键词:-agonists,residuals,QuEChERS,liquid chromatography-tandem mass spectrometry,
发表日期：2015-11-09
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