莱克多巴胺ELISA快检技术样品前处理
目的 建立适合莱克多巴胺ELISA快速检测技术基体干扰消除的样品前处理方法。方法 样品经乙腈?0.1 mol/L盐酸(84∶16, v/v)处理, 上清液采用正己烷萃取除杂, 水相用NaOH调成碱性后, 用乙酸乙酯萃取, 有机相层吹干后待测。结果 样品采用正己烷萃取1次后, 基体干扰可消除约25%; 萃取3次后, 样品中的基体干扰可被基本消除。结论 该前处理方法能有效消除莱克多巴胺快速检测的基体干扰, 对猪肝、猪肉等肉类食品的适用性良好。
Objective To establish an efficient sample pretreatment method for the rapid detection of ractopamine using ELISA technique. Methods The samples were extracted with acetonitrile and 0.1 mol/L HCl (84∶16, v/v), washed with n-hexane and then adjusted to basicity. The extract was further extracted with ethyl acetate and the ethyl acetate extractant was dried for further analysis. The influences of n-hexane, petroleum ether, n-heptane and cyclo-hexane were tested. Results Twenty five percentage of the matrix interferences were eliminated by extracting the sample one time with n-hexane, and the matrix interferences were eliminated after the sample was extracted 3 times. Conclusion This proposed sample pretreatment method is efficient and it has a good potential on the rapid detection of ractopamine in other meat samples.
标题：莱克多巴胺ELISA快检技术样品前处理
英文标题：Study on the sample pretreatment for rapid detection of ractopamine residues in foods by ELISA
作者：
肖小华 中山大学化学与化学工程学院
周思 中山大学化学与化学工程学院
李攻科 中山大学化学与化学工程学院
中文关键词:莱克多巴胺,ELISA,样品前处理,基体干扰,
英文关键词:ractopamine,ELISA,sample pretreatment,matrix interferences,
发表日期：2012-07-21
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