增敏荧光法测定蔬菜中丙烯菊酯的残留量
目的 建立蔬菜中丙烯菊酯残留量的增敏荧光测定方法。方法 β-环糊精对丙烯菊酯的荧光有增敏作用, 在加入1.0% β-环糊精溶液1.0 mL, Britton-Robinson(B-R)缓冲溶液(pH 6.75)1.0 mL, 40 ℃水浴30 min条件下进行测定。结果 丙烯菊酯在0.8~18 ng/mL浓度范围内与荧光强度呈良好的线性关系, 检出限为0.58 ng/mL。平均回收率为92.6%~95.0%, RSD为1.6%~2.4%。结论 该方法灵敏度高, 选择性好, 适合蔬菜中丙烯菊酯残留的测定。
Objective To establish a method for the determination of propylene chrysanthemum ester residues in vegetables by enhanced fluorimetry. Methods β-CD can enhance the fluorescence of propylene chrysanthemum ester. In the BR buffer medium of pH 6.75 and in the presence of β-CD, run for 30 min in 40 ℃ water bath, then propylene chrysanthemum ester was determined with fluorimetry. Results The linear relationship between fluorescence intensity and concentration of propylene chrysanthemum ester was achieved at the range of 0.8~18 ng/mL. The detection limit was 0.58 ng/mL with the rates of recovery being 92.6%~95.0%, and RSD being 1.6%~2.4%. Conclusion The method has a good sensitivity and selectivity, and therefore is suitable for the determination of propylene chrysanthemum ester residues in vegetables.
标题：增敏荧光法测定蔬菜中丙烯菊酯的残留量
英文标题：Determination of propylene chrysanthemum ester residues in vegetable by enhanced fluorimetry
作者：
董洁 忻州师范学院化学系
白岚云 忻州师范学院化学系
谢海婷 忻州师范学院化学系
李满秀 忻州师范学院化学系
中文关键词:丙烯菊酯,β-环糊精,增敏荧光法,
英文关键词:propylene chrysanthemum ester,β-CD,enhanced fluorimetry,
发表日期：2014-08-08
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