螺虫乙酯及其代谢物在番茄中的储藏稳定性

螺虫乙酯及其代谢物在番茄中的储藏稳定性
目的  建立超高效液相色谱-串联质谱法测定番茄样品中螺虫乙酯及其代谢产物残留的分析方法, 考察螺虫乙酯及其代谢产物在番茄中的储藏稳定性。方法  低温储藏0~274 d的番茄样品经乙腈提取, 采用NaCl和无水MgSO4除水, 离心过膜后的上清液经超高效液相色谱-串联质谱测定, 基质匹配标准溶液外标法定量。结果  螺虫乙酯及其4种代谢产物在0.001~0.5 mg/L范围内线性关系良好, 在0.02、0.1、1和5 mg/kg添加水平的平均回收率为75.1%~104.5%, 相对标准偏差小于11.93%(n=5)。番茄样品在–18 ℃储藏条件下螺虫乙酯、螺虫乙酯烯醇、螺虫乙酯羟基、螺虫乙酯醇酮的平均降解率均小于30%, 螺虫乙酯烯醇糖苷在储藏274 d时降解率大于30%(平均降解率53.3%)。结论  –18 ℃条件下螺虫乙酯、螺虫乙酯烯醇、螺虫乙酯羟基、螺虫乙酯醇酮在番茄样品中的储藏稳定期至少为274 d, 螺虫乙酯烯醇糖苷在番茄样品中储藏稳定期至少为182 d。

Objective  To establish a method for the determination of ethyl helminth and its metabolites in tomato samples by ultra performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS), and to investigate the storage stability of spirotetramat and its metabolites in tomato. Methods  Tomato samples stored at low temperature from 0 to 274 d were extracted by acetonitrile, and NaCl and anhydrous MgSO4 were used to remove water. The supernatant after centrifugation was determined by UPLC-MS/MS, and quantified by the matrix matching standard solution external standard method. Results  Spirotetramat and its 4 metabolites had a good linear relationship in the range of 0.001–0.5 mg/L, and the average recovery rates at the addition levels of 0.02, 0.1, 1 and 5 mg/kg were 75.1%–104.5%, the relative standard deviations were less than 11.93% (n=5). The average degradation rates of spirotetramat, BYI08330-enol, BYI08330-monohydroxy, BYI08330-ketohydroxy were less than 30% under the storage condition of tomato samples at –18 ℃. At storage time of 274 days, the degradation rate of BYI08330-enol-glucoside was more than 30% (average degradation rate was 53.3%). Conclusion  Under the conditions of –18 ℃, the storage stability of spirotetramat, BYI08330-enol, BYI08330-monohydroxy, BYI08330-ketohydroxy in tomato samples is at least 274 d, and the storage stability of BYI08330-enol-glucoside in tomato samples is at least 182 d.

标题：螺虫乙酯及其代谢物在番茄中的储藏稳定性
英文标题：Storage stability of spirotetramat and its metabolites in tomato

作者：
付岩 宁波市农业科学研究院
张莉 杭州宇航梦园农业科技有限公司
凌淑萍 宁波市农业科学研究院
王全胜 宁波市农业科学研究院
张亮 宁波市农业科学研究院
吴银良 宁波市农业科学研究院

中文关键词:螺虫乙酯及其代谢产物,番茄,超高效液相色谱-串联质谱法,储藏稳定性,
英文关键词:spirotetramat and its metabolites,tomato,ultra performance liquid chromatography-tandem mass spectrometry,storage stability,

发表日期：2020-03-23

2025-2-27 20:35 上传 螺虫乙酯及其代谢物在番茄中的储藏稳定性.pdf

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