传统明目中药材复配对视网膜损伤小鼠抗氧化的影响
目的 探讨各复配组方对视网膜损伤小鼠的影响。方法 将小鼠分为正常对照组、模型组、熟地黄+枸杞子组、熟地黄+菊花组、熟地黄+铁皮石斛组、熟地黄+枸杞子+菊花组共6组, 分别对小鼠连续干预8周后进行强光造模, 光照结束后用采集晶状体及肝脏组织进行抗氧化的指标检测。观察晶状体中谷胱甘肽过氧化物酶(glutathione peroxidase, GSH-Px)和总抗氧化能力(total antioxidant capacity, T-AOC), 肝组织中丙二醛(malondialdehyde, MDA)、超氧化物歧化酶(superoxide dismutase, SOD)、过氧化氢酶(catalase, CAT)、及GSH-Px的活力。结果 模型组晶状体GSH-Px与T-AOC值均显著低于正常对照组(P＜0.05); 各复配组GSH-Px和T-AOC值与模型组比较均显著升高(P＜0.05)。在肝脏抗氧化指标中, 模型组与正常组比较, MDA含量升高, SOD活力、CAT活力和GSH-Px活力下降; 与模型组比较, 各复配组均可降低MDA含量, 升高SOD活力、CAT活力和GSH-Px活力, 且均具有显著性差异(P＜0.05)。结论 各复配组均对光损伤造成的氧化损伤具有缓解作用。
Objective To investigate the effects of each compound prescription on retinal injury mice Methods The mice were divided into 6 groups: Normal control group, model group, Rehmanniae radix raeparata+Lycii fructus, Rehmanniae radix raeparata+Chrysanthemum, Rehmanniae radix raeparata+Dendrobium officinale, and Rehmanniae radix raeparata+Lycii fructus+Chrysanthemum. After 8 weeks of continuous intervention, the mice were subjected to strong light modeling. After the illumination was over, the lens and livers were used to detect the antioxidant indexes. The glutathione peroxidase (GSH-Px) and total antioxidant capacity (T-AOC) in the lens, and the malondialdehyde (MDA), superoxide dismutase (SOD), catalase (CAT), and GSH-Px in liver tissue were observed. Results The values of GSH-Px activity and T-AOC in the model group were significantly lower than those of the control group (P<0.05). The GSH-Px and T-AOC values of each compound group were significantly higher than those of the model group (P<0.05). From the results of the liver antioxidant index, compared with the control group, the MDA content of the model group increased, and the activities of SOD, CAT and GSH-Px decreased. Compared with model group, all the compound groups could reduce MDA content, increase SOD activity, CAT activity and GSH-Px activity, and each compound groups had significant differences (P<0.05). Conclusion All the compounds have alleviated the oxidative damage caused by light damage.
标题：传统明目中药材复配对视网膜损伤小鼠抗氧化的影响
英文标题：Effect of traditional Chinese medicine compound on the antioxidant retinal injury mice
作者：
王玥 天士力研究院
李丽维 天士力研究院
周王谊 天士力研究院
王丹 天津天士力(辽宁)制药有限责任公司
刘垚杰 天津科技大学食品科学与工程学院
徐咏全 天士力研究院
王浩 天津科技大学食品科学与工程学院
中文关键词:熟地黄,枸杞子,菊花,铁皮石斛,复配,光损伤,抗氧化,
英文关键词:Rehmanniae radix raeparata,Lycii fructus,Chrysanthemum,Dendrobium officinale,compound,light-damage,anti-oxidation,
发表日期：2020-05-15
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