气相色谱法分析超临界CO2萃取刺梨籽油的脂肪酸组成
目的 采用气相色谱法(gas chromatography, GC)对超临界CO2萃取得到的贵州产刺梨籽油进行脂肪酸组成分析。方法 刺梨籽油经脂肪酸甲酯化处理后, 以37种脂肪酸甲酯混合标准品为参照, 采用气相色谱法进行测量, 根据保留时间和峰面积归一化法对刺梨籽油的脂肪酸组成进行定性定量分析。结果 该刺梨籽油主要含有棕榈酸、硬脂酸、油酸、亚油酸、亚麻酸及花生酸6种脂肪酸, 以不饱和脂肪酸亚油酸、油酸、亚麻酸为主, 这3种脂肪酸含量占比高达90.97%, 其中n-6/n-3不饱和脂肪酸的比例为3.1:1。结论 刺梨籽油是一种富含不饱和脂肪酸, 且n-6/n-3脂肪酸比例合理、营养健康价值高的功能性油脂, 研究结果可为刺梨资源高附加值综合开发利用提供支撑。
Objective To analyze the fatty acid composition of Rosa roxburghii Tratt seed oil produced in Guizhou extracted by supercritical CO2 using gas chromatography (GC). Methods Rosa roxburghii seed oil was treated with fatty acid methyl ester, then the fatty acid components were determined by gas chromatography with a mixed standard with 37 kinds of fatty acid methyl used as reference. The qualitative and quantitative analysis of the fatty acid composition were carried out according to retention time and normalization method for peak area. Results The Rosa roxburghii seed oil mainly contained 6 fatty acids, including palmitic acid, stearic acid, oleic acid, linoleic acid, linolenic acid and arachidonic acid. It was mainly composed of unsaturated fatty acid linoleic acid, oleic acid and linolenic acid, and the content of these 3 main fatty acids together was 90.97%. The ratio of n-6/n-3 fatty acids was 3.1:1. Conclusion Rosa roxburghii Tratt seed oil is a kind of functional oil with reasonable n-6/n-3 fatty acid ratio and high nutritional and health value. These results can provide support for the comprehensive development and utilization of Rosa roxburghii Tratt resources.
标题：气相色谱法分析超临界CO2萃取刺梨籽油的脂肪酸组成
英文标题：Analysis on fatty acid composition of Rosa roxburghii Tratt seed oil extracted by supercritical CO2 using gas chromatography
作者：
滕桂平 贵州大学化学与化工学院；中国科学院地球化学研究所
唐泽群 贵州大学化学与化工学院；中国科学院地球化学研究所
田弋夫 中国科学院地球化学研究所
余德顺 贵州大学化学与化工学院；中国科学院地球化学研究所
中文关键词:刺梨籽油,超临界CO2,萃取,气相色谱法,脂肪酸,
英文关键词:Rosa roxburghii Tratt seed oil,supercritical CO2,extraction,gas chromatography,fatty acid,
发表日期：2020-05-15
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