Objective To establish a method for determination of total flavonoids in Brazilian green propolis powder by acidizing polyamide powder washing. Methods In the method, the acidizing polyamide powder is acidified to adsorb the total flavonoids, and then eluted with methanol. Prepare standard curve with acidified standard solution to determine the total flavonoids in the sample. Results The total flavonoid had a good linear relationship in the range of 2.137 to 21.370 μg/mL (r=0.99956), and the average recovery was 98.9% (RSD=0.7%, n=9). Conclusion The method can improve the elution efficiency and recovery rate of total flavonoids, it can improve the accuracy of the test results.
标题:酸化处理的聚酰胺粉方法测定巴西绿蜂胶粉中总黄酮
英文标题:Determination of total flavonoids in Brazilian green propolis powder by acidified polyamide powder