芜菁总多酚的提取工艺优化研究
目的 响应面法优化新疆芜菁中总多酚的提取工艺。方法 采用紫外分光光度法测定芜菁中总多酚的含量, 分别考察了乙醇浓度、料液比、超声时间、以及超声功率对芜菁总多酚得率的影响, 单因素的基础上进行了4因素3水平的设计, 以总多酚的得率为响应值, 分析数据, 优化新疆芜菁中总多酚的提取条件。结果 超声波提取新疆芜菁中总多酚的最佳工艺: 乙醇体积分数40%、固液比1: 30、时间30 min、功率 105 W时, 验证试验得出芜菁多酚的含量13.09 mg/g, 该值与预测值13.62 mg/g接近, 表明响应面法得到的工艺参数准确和可靠。结论 该方法提取时间短, 溶剂用量少, 提取效率高的优点, 可作为芜菁多酚提取工艺的一种有效手段。
Objective To optimize the extraction process of total polyphenols from Brassica rapa L. in xinjiang by response surface method. Methods The content of total polyphenols in Brassica rapa L. was determined by ultraviolet spectrophotometry. The effects of ethanol concentration, solid-liquid ratio, ultrasonic time and ultrasonic power on the yield of total phthalocyanine were investigated. On the basis of single factor, 4-factor and 3-level design was carried out, and the total polyphenols yield was taken as the response value to analyze the data and optimize the extraction conditions of total polyphenols in Brassica rapa L.. Results The optimal extraction process of total polyphenols in Brassica rapa L. by ultrasonic wave was as follows: ethanol volume fraction was 40%, solid-liquid ratio was 1:30, time was 30 min, power was 105 W. The results showed that the content of turnip polyphenols was 13.09 mg/g, which was close to the predicted value 13.62 mg/g, indicating that the process parameters obtained by response surface method were accurate and reliable. Conclusion The method has the advantages of short extraction time, less solvent consumption and high extraction efficiency, it can be used as an effective means for the extraction process of polyphenols from phthalocyanine.
标题：芜菁总多酚的提取工艺优化研究
英文标题：Optimization of extraction process of total polyphenols from Brassica rapa L.
作者：
海仁古丽·麦麦提 新疆医科大学药学院
刘展 新疆医科大学厚博学院
阿依夏古丽·巴卡斯 新疆医科大学药学院
海力茜·陶尔大洪 新疆医科大学药学院
中文关键词:芜菁,总多酚,响应面法,
英文关键词:Brassica rapa L.,total polyphony,response surface methodology,
发表日期：2019-06-24
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