液相微萃取-高效液相色谱法测定油脂中1, 4-特丁基对苯二酚
建立一种液相微萃取-高效液相色谱法测定油脂中1, 4-特丁基对苯二酚(t-butyl hydroquinone, TBHQ)的方法。方法 以0.1 g油脂为原料, 以400 μL 1, 4-丁二醇作为样品前处理萃取剂, 旋涡萃取2 min, 提取物采用高效液相色谱法(紫外检测)进行分析。结果 本方法线性关系良好, r2为1; 加标回收率较高(96.00%~114.67%), 日内和日间测定精密度良好(小于3%)。本方法应用于不同种类的食用油中TBHQ的测定时, 得到的测定结果与使用常规溶剂甲醇萃取前处理的结果相近。结论 该方法准确性高, 且效率高、时间短; 并且所需样品量小, 适合用于油脂中TBHQ的测定。
Objective To establish a method that determination of 1, 4- t-butyl hydroquinone (TBHQ) in edible oils by liquid phase microextraction-high performance liquid chromatography. Methods Using 0.1 g of fat as raw material and 400 μL of 1, 4-TBHQ as sample pretreatment extractant, the mixture was extracted by vortexing for 2 min, and the extract was analyzed by high performance liquid chromatography (ultraviolet detector). Results This established method showed good linearity (r2=1). The recoveries were in the range of 96.00%?114.67%, and the intra-day and inter-day precision were all less than 3.0%. When the method was applied to the determination of TBHQ in different kinds of edible oils, the obtained measurement results were similar to those obtained by the conventional solvent methanol extraction pretreatment. Conclusion The method has high accuracy, high efficiency and short time; and the required sample amount is small, which is suitable for the determination of TBHQ in fats and oils.
标题：液相微萃取-高效液相色谱法测定油脂中1, 4-特丁基对苯二酚
英文标题：Determination of 1, 4-t-butyl hydroquinone in edible oils by liquid phase microextraction-high performance liquid chromatography
作者：
张立新 河南省产品质量监督检验院
邹蕾 河南省产品质量监督检验院
宋君威 河南省产品质量监督检验院
王小迪 河南省产品质量监督检验院
中文关键词:抗氧化剂,特丁基对苯二酚,液相微萃取,高效液相色谱法,
英文关键词:antioxidants,1, 4-t-butyl hydroquinone,liquid-liquid microextraction,high performance liquid chromatography,
发表日期：2019-06-26
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