Objective To evaluate and compare the difference of uncertainty of determination of aflatoxin B1 in sesame sauce by 2 kinds of high performance liquid chromatograph. Methods The content of aflatoxin B1 in sesame sauce was determined by Agilent 1260 and Thermo U3000 high performance liquid chromatography, and the mathematical model of uncertainty analysis was used to analyze and quantify the sources of measurement uncertainty. Results The results (Agilent 1260, Thermo U3000) showed when the determination results of aflatoxin B1 were 6.1236 μg/kg and 6.0230 μg/kg, the extended uncertainties were 0.42706 μg/kg, 0.31115 μg/kg, and k=2. Conclusion The uncertainty introduced by the preparation of standard solution, the calculation of sample concentration by the least square method fitting standard curve and the determination of recovery has great influence on the results. Under this experimental condition, the relative uncertainty introduced by the Agilent 1260 measurement is greater than that introduced by the Thermo U3000 measurement, which is more stable and more accurate.
标题：2种高效液相色谱仪测定芝麻酱中黄曲霉毒素B1的不确定度评定
英文标题：Uncertainty evaluation for the determination of aflatoxin B1 in sesame sauce by 2 kinds of high performance liquid chromatograph
