超高效液相色谱-串联质谱法测定食品接触材料中油酸酰胺的迁移量
目的 建立超高效液相色谱-串联质谱法测定食品接触材料中油酸酰胺迁移量的分析方法。方法 按照GB 5009.156-2016《食品安全国家标准 食品接触材料及制品迁移试验预处理方法通则》的要求配制5种食品模拟液, 并按照标准要求进行浸泡试验, 浸泡液经过滤后进样, 经过ZORBAX C18 (3.0 mm×100 mm, 1.8 μm)色谱柱分离, 在正离子模式下以电喷雾电离多反应监测模式进行定性和定量分析。结果 5种食品模拟物(4%乙酸、10%乙醇、20%乙醇、50%乙醇以及95%乙醇)中的油酸酰胺在0.25~5.0 mg/L浓度范围具有较好线性, 相关系数(r2)均大于0.9990, 方法检出限为0.001~0.002 mg/L, 方法定量限为0.003~0.005 mg/L, 油酸酰胺在3个浓度水平的加标回收率为90.3%~117.1%, 相对标准偏差为0.20%~2.10% (n=6)。结论 该方法灵敏、准确, 能够满足食品接触材料中油酸酰胺的迁移量的检测。
Objective To establish a method for the determination of oleamide migration from food contact materials by ultra performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS). Methods According to the GB 5009.156-2016 National food safety standards-Food contact materials and products migration test pretreatment methods, 5 kinds of food simulation solutions were prepared. After the test of soaking, the solutions were detected by UPLC-MS/MS. Oleamide was separated by ZORBAX C18 (3.0 mm×100 mm, 1.8 μm) hromatographic column. The eluent was qualitatively and quantitatively determined by mass spectrometer with electrospray ionization in positive multiple reaction monitoring mode. Results Oleamide had good linear relationship in 5 kinds of food simulation solutions in the range of 0.25-5.0 mg/L with correlation coefficients more than 0.9990. The limits of detection of oleamide were 0.001-0.002 mg/L, the limit of quantification was 0.003-0.005 mg/L. The recoveries of oleamide at 3 spiked levels were 90.3%-117.1%, and the relative standard deviations (RSDs) were 0.20%-2.10% (n=6). Conclusion This method is accurate and reliable, which is suitable for the determination of oleamide migration in 5 kinds of food simulation solutions.
标题：超高效液相色谱-串联质谱法测定食品接触材料中油酸酰胺的迁移量
英文标题：Determination of oleamide migration from food contact materials by ultra performance liquid chromatography-tandem mass spectrometry
作者：
晏嫣 江苏省产品质量监督检验研究院,国家食品包装检测中心
张阳 江苏省产品质量监督检验研究院,国家食品包装检测中心
姜德玮 江苏省产品质量监督检验研究院,国家食品包装检测中心
向斌 江苏省产品质量监督检验研究院,国家食品包装检测中心
宋锦柱 江苏省产品质量监督检验研究院,国家食品包装检测中心
中文关键词:超高效液相色谱-串联质谱法,食品接触材料,油酸酰胺,食品模拟物,迁移,食品安全,
英文关键词:ultra performance liquid chromatography-tandem mass spectrometry,food contact materials,oleamide,food simulation,migration,food safety,
发表日期：2018-12-21
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