一种乳中钙含量的快速检测方法
目的 建立血液中钙离子临床快速检测方法检测乳中钙含量的方法。方法 样品采用盐酸+TX-100稀释, 依据碱性(酸性)条件下钙与偶氮胂Ⅲ作用生成的蓝色复合物颜色与钙浓度正比的正比关系, 在650 nm波长处测定吸光度变化, 经与钙校准液比较, 计算出样品中钙含量。方法的准确度、精密度、稳定性等用乳中钙含量检测国标方法进行验证。结果 建立的乳中钙含量快速测定方法与国标方法比较, 准确度(偏差≤10%的符合率达到100%)和精密度(相对标准偏差为10%)方面均符合要求, 且操作简单、成本低、安全性高, 单个样品检测时间由原来国标方法的24 h缩短至8 min。结论 该方法可应用于乳中钙含量快速检测, 对生乳采购和液态奶市场筛查检测具有重要的应用价值。
Objective To establish a method for the determination of calcium content in milk by rapid clinical detection of calcium ion in blood. Methods The sample was diluted with hydrochloric acid and TX-100. According to calcium and azo arsine Ⅲ color of blue complex and calcium concentration was directly proportional relationship under alkaline (acid) condition, the absorbance change was measured at the wavelength of 650 nm, and the calcium content in the sample was calculated by comparing with the calcium calibration solution. The accuracy, precision and stability of the method were verified by the national standard method for determination of calcium content in milk. Results Compared with the national standard method, the established method for the rapid determination of calcium content in milk met the requirements in terms of accuracy (the coincidence rate of deviation ≤10% reached 100%) and precision (the relative standard deviation was 10%). In addition, this method was simple to operate, low in cost and high in safety. The detection time of a single sample was shortened from 24 h in the original national standard method to 8 min. Conclusion This method can be applied to the rapid detection of calcium content in milk, which has important application value for raw milk purchase and liquid milk market screening.
标题：一种乳中钙含量的快速检测方法
英文标题：A rapid method for determination of calcium content in milk
作者：
梁春梅 内蒙古蒙牛乳业(集团)股份有限公司中心实验室
常建军 内蒙古蒙牛乳业(集团)股份有限公司中心实验室
喻东威 内蒙古蒙牛乳业(集团)股份有限公司中心实验室
宋晓东 内蒙古蒙牛乳业(集团)股份有限公司中心实验室
解鑫 内蒙古蒙牛乳业(集团)股份有限公司中心实验室
李照 内蒙古蒙牛乳业(集团)股份有限公司中心实验室
许璐 内蒙古蒙牛乳业(集团)股份有限公司中心实验室
吴玉秋 内蒙古蒙牛乳业(集团)股份有限公司中心实验室
中文关键词:乳钙快检方法,偶氮胂Ⅲ,国标方法,
英文关键词:milk calcium rapid detection method,ArsenazoⅢ,national standard method,
发表日期：2019-02-18
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