高效液相色谱-串联质谱法测定功能饮料中肌醇的含量
目的 建立液相色谱-串联质谱法测定功能饮料中肌醇含量。方法 功能饮料振荡混匀后取样0.2 mL，用50%的乙腈水稀释500倍，过0.22 μm的微孔滤膜后直接进样，经BEH Amide色谱柱分离，用串联四极杆质谱采用多反应监测模式进行测定，外标曲线法定量。结果 肌醇在0.05～2.0 μg/mL范围内线性关系良好，线性相关系数r为0.9999，检出限和定量限分别为0.65 mg/L和2.15 mg/L；加标浓度为0.025、0.125和0.25 mg/mL的平均回收率分别为103.5%、100.0%和101.8%，批内精密度分别为3.3%、1.1%和1.5%，批间精密度分别为2.4%、1.9%和2.1%。结论 该方法前处理简单、快速、准确、灵敏，可以满足实际检测分析的需要。
Objective To establish a method for the determination of inositol in functional drink by high performance liquid chromatography-tandem mass spectrometry. Methods After shaking and mixing, the functional drink was diluted 500 times with 50% acetonitrile water, and directly injected after passing through 0.22 μm microporous membrane filter. Inositol was separated by BEH Amide column, determined by tandem quadrupole mass spectrometry using multi-reaction monitoring mode, and quantified by external standard method. Results The inositol had a good linear relationship in the concentration range of 0.05-2.0 μg/mL with correlation coefficients of 0.9999. The detection limit and quantitative limit were 0.65 mg/L and 2.15 mg/L, respectively. The average recovery of inositol at 0.025, 0.125 and 0.25 mg/mL were 103.5%, 100.0% and 101.8%, respectively, and intra-batch precision were 3.3%, 1.1% and 1.5%, respectively，inter-batch precision were 2.4%, 1.9% and 2.1%, respectively. Conclusion The method is simple, accurate and sensitive which can meet the requirements of practical analysis.
标题：高效液相色谱-串联质谱法测定功能饮料中肌醇的含量
英文标题：Determination of inositol in functional drink by high performance liquid chromatography with tandem mass spectrometry
作者：
黄传峰 中国食品药品检定研究院
张会亮 中国食品药品检定研究院
房子舒 中国食品药品检定研究院
孙晶 山东省食品药品检验研究院
林思静 中国食品药品检定研究院
王海燕 中国食品药品检定研究院
曹进 中国食品药品检定研究院
丁宏 中国食品药品检定研究院
中文关键词:肌醇,功能饮料,高效液相色谱-串联质谱,
英文关键词:inositol,functional drink,high performance liquid chromatography-tandem mass spectrometry,
发表日期：2018-12-12
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