Objective The real-time PCR was used to assist test the Salmonella in proficiency testing as to improve accuracy. Methods The samples were tested by traditional methods of enrichment, selective culture and biochemical identification. The real-time fluorescent PCR as an assistant method to amplify BPW enrichment solution and suspicious colonies. Finally, suspicious colonies were identified by API20E identification system. Results No suspicious colonies were found in the three samples after enrichment, selective culture and biochemical identification. Real-time fluorescent PCR amplification with BPW enrichment solution showed that sample S02 was suspicious and the colony S02-4 isolated from the sample S02 had typical amplification curve. Finally the API20E identification system confirmed that the sample S02 was positive and the suspicious bacteria were Salmonella cholerae subspecies Arizona. Conclusions The real-time PCR is simple to operate with high accuracy and can be used as an effective complement to the traditional testing methods.
标题：运用实时荧光PCR法辅助鉴定能力验证样品中的沙门氏菌
英文标题：Aided test the Salmonella in the samples of proficiency testing by real-time fluorescent PCR
