高效液相色谱法检测葡萄酒中柠檬酸
目的 建立高效液相色谱法(high performance liquid chromatography, HPLC)测定葡萄酒中柠檬酸的含量的分析方法。方法 以0.02 mol/L甲醇和磷酸二氢钾(5:95, V:V)为流动相, 流速为0.7 mL/min, 在此色谱条件下, 采用二级管阵列检测器进行检测。结果 在0.05~1.00 g/L范围内, 浓度与峰面积的线性关系良好, 相关系数为0.9999。在加标浓度为5.0、20.0、50.0 g/L时的回收率为93.9%~106.3%, 相对标准偏差(relative standard deviation, RSD)为1.67%~2.99%之间。结论 该方法具有较高的灵敏度和准确性, 且操作简单, 适用于检测葡萄酒样品中的柠檬酸。
Objective To establish a method for determination of the content of citric acid in wine by high performance liquid chromatography method (HPLC). Methods The mobile phase was 0.02 mol/L methanol and potassium dihydrogen phosphate (5:95, V:V), and the flow rate was 0.7 mL/min. The sample was detected by the secondary tube array detector. Results In the range of 0.05~1.00 g/L, the linear relationship between the concentration of citric acid and the peak area was good, and the correlation coefficient was 0.9999. At the spiked levels of 5, 20, and 50 g/L, the recovery rates were 93.9%-106.3%, and the relative standard deviation (RSD) was 1.67%-2.99%. Conclusion This method has high sensitivity and accuracy, and simple operation, which is suitable for detecting citric acid in wine samples.
标题：高效液相色谱法检测葡萄酒中柠檬酸
英文标题：Determination of citric acid in wine by high performance liquid chromatography
作者：
何军风 平顶山市质量技术监督检验测试中心
中文关键词:高效液相色谱法,葡萄酒,柠檬酸,
英文关键词:high performance liquid chromatography,wine,citric acid,
发表日期：2018-01-30
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