高效液相色谱法测定不同产地湘莲中荷叶碱、 莲心碱、芦丁的含量
目的 建立高效液相色谱法同时测定湘莲中荷叶碱、莲心碱、芦丁的方法。方法 在酸性条件下超声提取3种待测组分。色谱条件如下: C18(250 mm×4.6 mm, 5 μm)色谱柱; 流动相: 甲醇-0.1 mol/L乙酸铵(用乙二胺调节pH值至7.5~8.0)(55:45, V:V); 流速: 1.0 mL/min; 检测波长: 275 nm; 柱温30 ℃; 进样量10 μL。 结果 荷叶碱、莲心碱、芦丁分别在7.636~152.7 μg/mL(r2=0.9999, n=5)、7.543~150.9 μg/mL(r2=0.9995, n=5)、7.543~150.9 μg/mL(r2=0.9998, n=5)范围内线性关系良好, 加标回收率为96.9%~100.5%之间, 相对标准偏差(relative standard deviation, RSD)为1.1%~1.4%。结论 该法同时测定湘莲中荷叶碱、莲心碱、芦丁, 简单快速, 定量准确, 可以为评价不同产地湘莲功效成分提供参考。
Objective To establish a method for simultaneous determination of nuciferine, liensinine and rutin in Hunan lotus seeds by high performance liquid chromatography. Methods Totally 3 kinds of components were extracted by ultrasonic under acid condition. The chromatographic conditions were as follows: column was C18 (250 mm×4.6 mm, 5 μm), methanol-0.1 mol/L ammonium acetate (to adjust the pH value to 7.5~8.0 with ethylenediamine) (55:45, V:V) was as mobile phase, flow rate was 1 mL/min, detection wavelength was 275 nm, column temperature was 30 ℃ and sample size was 10 μL. Results The nuciferine, liensinine and rutin showed a good linear range at 7.636~152.7 μg/mL (r2=0.9999, n=5), 7.543~150.9 μg/mL (r2=0.9995, n=5) and 7.543~150.9 μg/mL (r2=0.9998, n=5), respectively. The recoveries were 96.9%~100.5% at the spiked levels with relative standard deviation of 1.1%~1.4%. Conclusion This method is accurate, simple and reliable, and it provides a reference for the evaluation of different origin of lotus ingredients, which can be applied to the simultaneous determination of nuciferine, liensinine and rutin in lotus seeds.
标题：高效液相色谱法测定不同产地湘莲中荷叶碱、 莲心碱、芦丁的含量
英文标题：Determination of nuciferine, liensinine, rutin in Hunan lotus seeds from different origins by high performance liquid chromatography
作者：
唐湘伟 邵阳市食品药品检验所
中文关键词:湘莲,荷叶碱,莲心碱,芦丁,高效液相色谱法,
英文关键词:Hunan lotus seeds,nuciferine,liensinine,rutin,high performance liquid chromatography,
发表日期：2018-01-10
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