Objective To establish a rapid, specific and sensitive method for the detection of duck derived components by droplet digital PCR. Methods According to the whole sequence of duck mitochondrial gene, primers and probes were designed by droplet digital PCR to establish a method for detection of duck source components. The specific detection of droplet digital PCR was carried out with the reference species of common livestock and poultry meat in the market. The sensitivity of the method was detected by gradient dilution of duck meat DNA with mutton DNA as interference. Results The method was effective and specific for the detection of duck origin components. And the presence of other species had no effect on the sensitivity of the method. Conclusion This method has strong specificity and high sensitivity, which can be used to detect duck origin in animal meat food.
标题：微滴数字PCR方法检测畜肉食品中鸭源性成分
英文标题：Detection of duck-derived materials in meat products by droplet digital PCR
