Objective To prepare a new solid structure stationary phase and establish a new method for separation of 7 organic acids by high performance liquid chromatography (HPLC). Methods Totally 7 organic acids were separated by new solid structure stationary phase with the 5% methanol-95% 10 mmol/L ammonium formate (pH=3.0) solution as a mobile phase for isocratic elution. Flow rate was 1.0 mL/min, column temperature was 40 ℃, detection wavelength was 220 nm and injection volume was 10 μL. Then the samples were detected by HPLC. Results A new solid structure stationary phase was prepared, which had hydrophobic effect similar to that of C18 stationary phase by new solid structure stationary phase. Totally 7 organic acids were completely separated within 8 min, and the separation effect was better. Conclusions The established method has the advantages of strong retention, rapid determination and good separation efficiency, which is suitable for detection of many organic acids.
标题：基于新型立体结构的色谱固定相的高效液相色谱法检测分离7种有机酸
英文标题：Separation and development of 7 organic acids by high performance liquid chromatography with a new solid structure stationary phase
