高效液相色谱法测定食品中胭脂红酸
目的 建立高效液相色谱法测定食品中胭脂红酸含量的分析方法。方法 用2 mol/L盐酸提取样品中的胭脂红酸, 经混合型阴离子交换固相萃取柱净化后, 以0.02 mol/L磷酸二氢铵和甲醇作为流动相, 采用二极管阵列检测器进行检测。结果 胭脂红酸在0.2~100 μg/mL的范围内, 浓度与峰面积的线性关系良好, 相关系数(r2)为0.9999; 方法检出限为0.3 mg/kg, 方法定量限为1.0 mg/kg, 在10、50、200 mg/kg 3个添加水平时, 平均加标回收率为84.8%~97.3%(n=6), 相对标准偏差为2.7%~7.9%。结论 该方法操作简单, 具有较高的灵敏度和准确性, 适用于食品中胭脂红酸的测定。
Objective To establish a method for the determination of carmine acid in food by high performance liquid chromatography (HPLC). Methods The sample was extracted by 2 mol/L hydrochloric acid, and purified by mixed anion exchange solid phase extraction column. The mobile phase were 0.02 mol/L ammonium dihydrogen phosphate and methanol, and the samples were detected by diode array detector. Results Carminic acid had good linear relationship in the range of 0.2-100 μg/mL, and the correlation coefficient (r) was 0.9999. The limit of detection and limit of quantitation were 0.3 mg/kg and 1.0 mg/kg, respectively. The recoveries at 3 spiked levels (10, 50, 200 mg/kg) were 84.8%-97.3%, with the relative standard deviations (RSDs) were 2.7%-7.9%. Conclusion This method is simple to operate, and has the advantage of high sensitivity and accuracy, which is suitable for the determination of carmine red acid in food.
标题：高效液相色谱法测定食品中胭脂红酸
英文标题：Determination of carminic acid in foods by high performance liquid chromatography
作者：
陈营寿 湛江市食品药品检验所
刘唤明 广东海洋大学食品科技学院
高平 湛江市食品药品检验所
陈康健 湛江恒兴水产科技有限公司
中文关键词:高效液相色谱法,胭脂红酸,固相萃取,
英文关键词:high performance liquid chromatography,carminic acid,solid phase extraction,
发表日期：2018-09-26
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