高效液相色谱法测定营养强化剂中氯化高铁血红素的含量
目的 建立高效液相色谱法(high performance chromatography, HPLC)测定营养强化剂中氯化高铁血红素的含量。方法 采用0.1 mol/L氢氧化钠溶液提取样品, 以Agilent TC-C18为色谱分析柱, 流动相为0.6%乙酸:甲醇=30:70(V:V), 流速1.0 mL/min, 柱温35 ℃, 检测波长399 nm, 采用高效液相色谱-二极管阵列检测器进行检测, 以峰面积外标法定量。结果 该方法在40~140 mg/L范围内线性关系良好, 相关系数r为0.9993, 相对标准偏差(relative standard deviation, RSD)小于1.38%。结论 该方法操作简单、快速、准确、灵敏度高, 适合测定营养强化剂中氯化高铁血红素的含量。
Objective To establish a method for determination of hemin in nutrient supplement by high performance chromatography (HPLC). Method The sample was extracted with 0.1 mol/L sodium hydroxide solution, and was analyzed with Agilent TC-C18. The mobile phase was 0.6% acetic acid: methanol=30:70 (V:V), the flow rate was 1.0 mL/min, and the column temperature was 35 ℃. The detection wavelength was 399 nm, and the detection was performed by a high performance liquid chromatography-diode array detector. And the samples were quantified by peak area external standard method. Result The method had a good linear relationship in the range of 40-140 mg/L, the correlation coefficient r was 0.9993, and the relative standard deviations (RSDs) were less than 1.38%. Conclusion The method is simple, rapid, accurate and sensitive, which is suitable for the determination of hemin in nutrient supplement.
标题：高效液相色谱法测定营养强化剂中氯化高铁血红素的含量
英文标题：Determination of hemin in nutrient supplement by high performance liquid chromatography
作者：
钟钰 广东出入境检验检疫局技术中心
林海丹 广东出入境检验检疫局技术中心
官咏仪 广东出入境检验检疫局技术中心
张美金 广东出入境检验检疫局技术中心
金梦 广东出入境检验检疫局技术中心
陈毓芳 广东出入境检验检疫局技术中心
宋阳 广东出入境检验检疫局技术中心
中文关键词:营养强化剂,氯化高铁血红素,高效液相色谱法,
英文关键词:nutrient supplement, hemin, high performance chromatography,
发表日期：2018-01-05
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