Objective To evaluate the uncertainty of thiamphenicol residues in milk by high performance liquid chromatography (HPLC). Methods According to GB29689-2013 National standard for food safety-Determination of thiamphenicol residues in milk-High performance liquid chromatography and JJF 1059.1-2012 Measurement and expression of measurement uncertainty, the mathematical model for the determination of the uncertainty of thiamphenicol residues in milk by HPLC was established. The uncertainty of the fitting of the standard curve of thiamphenicol was assessed by least squares method by analyzing and synthesizing the sources of uncertainty throughout the assay. Results When the sample was 5.00 g, k=2 (95% confidence), the result of determination of the residual amount of thiamphenicol in milk with a theoretical content of thiamphenicol of 100 μg/kg was (91.4± 2.2) μg/kg. Conclusion The main sources of uncertainty for this method include standard solution concentration, standard curve fit, and sample measurement repeatability.
标题：高效液相色谱法测定牛奶中甲砜霉素残留量的不确定度评定
英文标题：Uncertainty evaluation of the determination of thiamphenicol residues in milk by high performance liquid chromatography
