气相色谱-质谱法测定人体血液中的毒鼠强
目的 建立气相色谱-质谱(gas chromatography-mass spectrometry, GC-MS)测定人体血液中的毒鼠强的方法。方法 血液样品用乙酸乙酯提取后, 用GC-MS进行测定。通过单离子检测扫描模式(single ion monitoring, SIM)确定毒鼠强的特征离子进行定性, 并以毒鼠强的3个特征离子(m/z121、m/z212、m/z240)作为母离子, 使用不同的碰撞能进行碰撞, 得到相应的子离子。选择分别来自3个母离子的离子对, 以响应值较高的离子对作为定量离子对, 其余2个离子对作为定性离子对, 采样选择反应监测(selective reaction monitoring, SRM)方式进行测定。结果 毒鼠强浓度范围在0.01~0.2 μg/mL范围内呈正相关, 相关系数r为0.9998, 回收率为92.0%~104.5%, 相对标准偏差(relative standard deviation, RSD)为3.62%~5.32%, 血液样品的检出限为0.0005 μg/mL。结论 该方法灵敏度高, 选择性好, 检出限低, 适合食物中毒人体血液中毒鼠强的痕量检测。
Objective To establish a method for determination of tetramine in human blood by gas chromatography-mass spectrometry (GC-MS). Methods The blood sample was extracted with ethyl acetate, and determined by GC-MS. The characteristic ions of tetramine were determined by single ion monitoring (SIM), and three characteristic ions (m/z121, m/z212 and m/z240) of tetramine were used as the parent ions, and different collision energy was used to collide. Ion pairs from 3 parent ions were selected, and ion pairs with higher response values were used as quantitative ion pairs. The other 2 ion pairs were used as qualitative ion pairs, and the selective reaction monitoring (SRM) method was used for measurement. Results There was a positive correlation between the concentration of tetramine in the range of 0.01-0.2 μg/mL, with the correlation coefficient (r) of 0.9998. The recoveries were 92.0%-104.5%, relative standard deviation (RSD) were 3.62%-5.32%, and the limit of detection of blood samples was 0.0005 μg/mL. Conclusion This method has high sensitivity, good selectivity and low detection limit, which is suitable for trace detection of tetramine in human blood.
标题：气相色谱-质谱法测定人体血液中的毒鼠强
英文标题：Determination of tetramine in human blood by gas chromatography-mass spectrometry
作者：
赵丽 昆明市疾病预防控制中心
段毅宏 云南省疾病预防控制中心
师真 昆明市疾病预防控制中心
李彦生 云南省疾病预防控制中心
刘建辉 云南省疾病预防控制中心
中文关键词:毒鼠强,人体血液,食物中毒,气相色谱-质谱法,
英文关键词:tetramine,human blood,food poisoning,gas chromatography-mass spectrometry,
发表日期：2018-06-27
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