液相色谱-串联质谱法测定肉鱼禽产品中的防腐剂月桂酰精氨酸乙酯盐酸盐
目的 建立液相色谱串联质谱法测定肉鱼禽产品中的防腐剂月桂酰精氨酸乙酯盐酸盐(ethyl lauroyl arginine hydrochloride, LAE)的分析方法。方法 通过超声波溶剂提取法提取样品中的LAE, 通过色谱柱筛选确定Waters ACQUITY UPLC?BEH C18(2.1 mm×100 mm, 1.7 μm)色谱柱, 流动相为0.1%甲酸水溶液和乙腈, 梯度洗脱, 然后经质谱法检测, 以外标法定量。结果 最佳提取条件为: 提取溶剂为水:乙腈=1:9(V:V), 提取时间为5 min, 提取次数为3次。LAE的线性范围为0~20 μg/L, 线性相关系数为0.9990。方法的检出限为0.3 μg/kg, 方法的定量限为1.0 μg/kg; 通过加标回收率实验, 确定方法的加标回收率范围为82.5%~105.0%, 符合GB27404-2008标准规定。结论 本方法简单、便捷, 适用于肉鱼禽产品中LAE含量的测定。
Objective To establish a method for detection of ethynl lauroyl arginate hydrochloride (LAE) in meat, fish and poultry products by liquid chromatography-tandem mass spectrometry (LC-MS/MS). Methods LAE was extracted by ultrasonic from samples, separated by an ACQUITY UPLC? BEH C18(2.1 mm×100 mm, 1.7 μm) column using 0.1% formic acid water solution-acetonitrile as mobile phase for gradient elution and then detected by tandem mass spectrometry with external standard method for quantification. Results The optimum extracting conditions were as follow: water: acetonitrile was 1:9 (V:V), extracting time was 5 min, and extraction times was 3 times. LAE had a good linearity in the range of 0~20 μg/L (r=0.9990). The limit of detection was 0.3 μg/kg and the limit of quantification was 1.0 μg/kg. The recovery rates were ranged from 85.2% to 105.0%, which were in line with GB27404-2008 standards. Conclusion This method is simple and convenient, which is suitable for the detection of LAE in the products of meat, fish and poultry.
标题：液相色谱-串联质谱法测定肉鱼禽产品中的防腐剂月桂酰精氨酸乙酯盐酸盐
英文标题：Determination of ethyl lauroyl arginate hydrochloride in meat, fish and poultry products by liquid chromatography-tandem mass spectrometry
作者：
赵艳菊 上海市质量监督检验技术研究院
杨晋青 上海市质量监督检验技术研究院
聂磊 上海市质量监督检验技术研究院
杨保刚 上海市质量监督检验技术研究院
周耀斌 上海市质量监督检验技术研究院
中文关键词:月桂酰精氨酸乙酯盐酸盐,液相色谱串联质谱法,溶剂提取,
英文关键词:ethyl lauroyl arginine hydrochloride,liquid chromatography-tandem mass spectrometry,solvent extraction,
发表日期：2017-02-20
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