Objective To optimize the microwave-assisted extraction (MAE) process of polysaccharide from Lentinus edods root (LRP), and study its antioxidant activity. Methods Single factor experiments involving three technological parameters including extraction time, microwave power and material/solvent ratio were selected for following investigations on the interactive effects of the parameters on LRP yield using central composite design combined with response surface methodology. A quadratic polynomial regression model was developed for LRP yield as a response to the above parameters and then the optimal extraction process conditions were obtained. The methods of DPPH and Fenton reaction were applied for determination of antioxidant activity. Results The optimal conditions of MAE were as follows: extraction time was 8 min, microwave power was 400 W and material/solvent ratio was 1:7 (m:V), and the yield of LRP was up to 4.91%. The antioxidant assays showed that the DPPH and the hydroxyl radical scavenging activities of extracts were strong. Conclusion The present study can provide a reference for reuse and development of LRP.
标题：响应曲面法优化微波辅助提取香菇柄多糖及其 抗氧化活性研究
英文标题：Optimization of microwave-assisted extraction of polysaccharides from Lentinus edods root by response surface analysis and its antioxidant activity
