胀果甘草酸性多糖的分离纯化、结构分析及免疫活性测定
目的 分离纯化胀果甘草多糖, 并对得到的3种酸性多糖组分结构及免疫活性进行分析。方法 采用水提醇沉法提取胀果甘草粗多糖, 经离子交换柱层析和凝胶柱层析分离纯化, 高效凝胶渗透色谱法(high performance gel permeation chromatography, HPGPC)、红外光谱法(infrared spectroscopy, IR)、气相色谱法(gas chromatography, GC)和气相-质谱法(gas chromatography-mass spectrometry, GC-MS)分析其结构, 采用H3-胸腺嘧啶核苷掺入法(H3-TdR)测定其对小鼠淋巴细胞增殖的影响。结果 胀果甘草根及根茎经水提醇沉、离子交换柱层析和凝胶柱层析分离纯化后, 得到3种相对分子量大于2.0×106 Da的均一酸性多糖组分GiP-B1、GiP-C1和GiP-D1。结构和免疫活性的研究结果表明, 这3种多糖组分均是由阿拉伯糖(Ara)、鼠李糖(Rha)、半乳糖(Gal)、半乳糖醛酸(GalA)以不同摩尔比组成的酸性杂多糖, 多糖的主链均由1,4-GalpA和1,2-Rhap残基构成, 支链由1,5-连接的Araf和1,3-连接的Galp构成, 支链分支点位于Rhap残基的O-4位。3种多糖在体外对小鼠脾细胞增殖均有促进作用, 与空白对照组均有显著性差异(P<0.05), 其中糖醛酸含量最高的GiP-D1促脾细胞增殖作用最高。结论 从胀果甘草中分离纯化的3种酸性多糖为均一组分, 具有一定的免疫增强活性。
Objective To isolate and purify polysaccharide fractions from Glycyrrhiza inflata Bat. and analyze their basic chemical structures and immune activities. Methods Crude polysaccharides were extracted using water extracting-alcohol precipitating method, and sequentially purified by ion-exchange and gel column chromatography. Their basic chemical structures were analyzed the methods, including HPGPC (high performance gel permeation chromatography), IR(infrared spectroscopy), GC(gas chromatography) and GC-MS(gas chromatography-mass spectrometry). Their immune activities were evaluated by the H3-TdR assay. Results Three acid homogeneous polysaccharides (GiP-B1, GiP-C1, GiP-D1) were purified by ion-exchange and gel column chromatography. The molecular weights of 3 homogeneous polysaccharides were over 2.0×106 Da. They were composed of arabinose, rhamnose, galactose and galacturonic acid with different molar ratio. They might have the similar structure characteristics: the backbone was mainly made up of 1,4-linked-α-GalpA and 1,2-linked-α-Rhap residues, and the side chains were composed of 1,5-linked-α-Araf residues and 1,3-linked-α-Galp residues, mainly linked at the O-4 of α-Rhap. The results of H3-TdR assay showed that certain concentrations of 3 homogeneous polysaccharides could promote the proliferation of lymphocyte and there were significant differences (P<0.05) compared with the blank control. Conclusion GiP-B1, GiP-C1 and GiP-D1 extracted from Glycyrrhiza inflata Bat. are homogeneous polysaccharides and they have effects on enhancing immune responses.
标题：胀果甘草酸性多糖的分离纯化、结构分析及免疫活性测定
英文标题：Isolation, purification, structural analysis and immune activity determination of acid polysaccharides from Glycyrrhiza inflata Bat.
作者：
陈橙 新疆医科大学药学院
帕丽达•阿不力孜 新疆医科大学药学院
米仁沙•牙库甫 新疆医科大学药学院
丛媛媛 新疆医科大学药学院
中文关键词:胀果甘草,多糖,分离纯化,结构分析,免疫活性,
英文关键词:Glycyrrhiza inflata Bat.,polysaccharide,isolation and purification,structural analysis,immune activity,
发表日期：2017-10-29
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