Objective To investigate the application of film chip technology for detection of animal-derived components in meat and its products, and to verify the accuracy and feasibility of the technology. Methods Animal-derived components in meat products were detected by using film chip, the sensitivity and limit of detection (LOD) for components of Sus scrofa domestica, Bos mutus, Equus asinus and Ovis aries were tested in samples, and the detection results of actual samples by film chip were compared with those by the national standards. Results The technique had good specificity for detection of animal-derived components. The sensitivities of the technology was 0.1 ng and the LODs were 0.1% (w:w) for the 4 kinds of animal-derived components. The technology was applicable to a wide range of samples. The test results of the actual samples by film chip were in accordance with those by national standard of fluorescence PCR method. Conclusion This technique can be used as a rapid screening method, which provides theoretical basis for adulteration detection and identification of animal-derived components in meat products.
标题:膜芯片用于肉制品中动物源性成分检测的研究
英文标题:Application of film chip for detection of animal-derived components in meat products