ELISA法快速检测婴儿配方奶粉中酪蛋白含量
目的 建立快速检测奶粉中酪蛋白含量的方法。方法 通过夹心酶联免疫吸附法(ELISA)定量测定婴儿配方奶粉中的酪蛋白含量。按照试剂盒内附的操作说明, 制作标准曲线,计算3种奶粉样品所对应的酪蛋白含量, 同时测量另外10种奶粉样品的酪蛋白含量。结果 酪蛋白在0.5~13.5 μg/g内具有良好的线性关系, 相关系数(R2)大于0.99。3种奶粉样品实际测得酪蛋白含量与标签上的含量一致性在99%以上。10种奶粉样品酪蛋白含量在4.17~6.65 g/100 g范围内。结论 该方法专一性强, 灵敏度高, 简便、快速、准确, 可用于婴儿配方奶粉中的酪蛋白含量的测定。
Objective To establish a method for the rapid detection of casein content in milk powder. Methods The content of casein in infant formula was quantitatively determined by enzyme-linked immunosorbent assay (ELISA). In accordance with the reference of the kit, a standard curve was made, the casein content of three different kinds of samples were calculated, and another 10 kinds of milk powder were detected. Results Casein had a good linear relationship in the range of 0.5~13.5 μg/g, with the correlation coefficient (R2) greater than 0.99. The consistency between detected casein content and label content in 3 kinds of milk powder samples were above 99%. The casein content of 10 kinds of milk powder samples were in the range of 4.17~6.65 g/100 g. Conclusion This method is specific, sensitive, simple, rapid and accurate, which is suitable for the determination of casein content in infant formula.
标题：ELISA法快速检测婴儿配方奶粉中酪蛋白含量
英文标题：Detection of casein content in infant formula by ELISA
作者：
于艳丽 上海杉达学院
蔡松铃 上海杉达学院
中文关键词:奶粉,夹心酶联免疫吸附法,酪蛋白,快速检测试剂盒,
英文关键词:milk powder,enzyme-linked immunosorbent assay,casein,rapid detection kit,
发表日期：2017-06-23
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