QuEChERS-超高效液相色谱-串联质谱法快速测定黄豆芽中的6-苄基腺嘌呤
目的 建立黄豆芽中的6-苄基腺嘌呤的超高效液相色谱-串联四极杆质谱测定方法。方法 黄豆芽样品经乙腈超声提取后, 经C18和PSA的固相分散净化后进样分析。使用SB-Aq色谱柱分离, 以水-乙腈为流动相进行梯度洗脱。采用电喷雾离子化源, 以多反应监测(multiple reaction monitoring)方式分别在负离子模式下进行检测。结果 6-苄基腺嘌呤的线性范围为5~200 μg /kg, 相关系数良好, 检出限为0.5 μg/kg, 该方法在三个水平上添加回收率在88.1%~107%, 相对标准偏差在1.4%~13.4%。结论 本方法灵敏度高, 操作简单高效, 适合于黄豆芽中的6-苄基腺嘌呤的定量及确证分析。
Objective To establish an ultra-high pressure liquid chromatography (UHPLC) coupled to tandem mass spectrometry (MS/MS)method for the determination of 6-benzylaminopurine in soybean sprouts. Methods The analyte was extracted from homogenized soybean sprouts samples with acetonitrile, and solid phase dispersed by C18 and PSA. The chromatography separation was performed on a SB-Aq column by ace-tonitrile and water as mobile phase in gradient elution program．Then samples were determined by electrospray ionization in negative mode using multiple reactions monitoring(MRM). Results A good linear relationship was obtained in range of 5~200 μg /kg, the limit of detection was 0.5 μg/kg. The recoveries at 3 spiked levels were between 88.1%~107%, with the relative standard deviations (RSDs) between 1.4%~13.4% (n=6). Conclusion The method was sensitive, effective, and simple, and was suitable for the determination and confirmation of 6-benzylaminopurine in soybean sprouts.
标题：QuEChERS-超高效液相色谱-串联质谱法快速测定黄豆芽中的6-苄基腺嘌呤
英文标题：Determination of 6-benzylaminopurine in soybean sprouts by QuEChERS-UHPLC-MS/MS method
作者：
米玛卓玛 西藏山南地区疾病预防方控制中心
达瓦曲珍 西藏山南地区疾病预防方控制中心
孔芳 应用毒理湖北省重点实验室,湖北省疾病预防控制中心
李永刚 湖北省疾病预防控制中心应用毒理湖北省重点实验室,湖北省疾病预防控制中心
罗苹 应用毒理湖北省重点实验室,湖北省疾病预防控制中心
刘小红 应用毒理湖北省重点实验室,湖北省疾病预防控制中心
德吉宗巴 西藏山南地区疾病预防方控制中心
中文关键词:QuEChERS,超高效液相色谱-串联四极杆质谱法,黄豆芽,6-苄基腺嘌,
英文关键词:QuEChERS,high performance liquid chromatography-tandem mass spectrometry,soybean sprouts,6-benzylaminopurine,
发表日期：2015-10-08
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