多种蜂胶中总黄酮检测方法的适用性研究
目的 通过3种分光光度法之间差异的比较和适用性考量, 筛选出适合检测蜂胶中总黄酮含量的分光光度法。方法 采用同批次样品, 对蜂胶(国产蜂胶和巴西绿蜂胶)中总黄酮检测方法进行回收率和含量测定, 并对结果进行分析。结果 巴西绿蜂胶粉3种方法的检测结果为4.99~9.34 g/100 g; 国产蜂胶粉3种方法的检测结果为7.82~17.97 g/100 g, 其RSD为24.2%~31.2%; 可见3种方法测定结果偏差较大。结论 GB/T 20574-2006的方法最适合蜂胶中总黄酮的检测。
Objective To choose the best method for detection of the total flavone content in propolis by comparing the differences of 3 spectrophotometry methods. Methods The content of total flavone in the samples (domestic propolis and Brazilian green propolis) from the same batch was determined by the 3 different methods respectively and the results were analyzed. Results By different methods, the content of total flavone was 4.99~9.34 g/100 g in green propolis, and 7.82~17.97 g/100 g in domestic propolis, with the RSD of 24.2%~31.2%. There was a big deviation among the 3 methods. Conclusion The method from GB/T 20574-2006 is the best one to determine the total flavone content in propolis.
标题：多种蜂胶中总黄酮检测方法的适用性研究
英文标题：Applicability of methods for detection of total flavone in multiple propolis
作者：
杨亚飞 汤臣倍健股份有限公司
胡烨敏 广州市宜健医学技术发展有限公司
钱蔚 汤臣倍健股份有限公司
曾燕媚 广州市宜健医学技术发展有限公司
游景水 汤臣倍健股份有限公司
杨祖伟 汤臣倍健股份有限公司
中文关键词:蜂胶,总黄酮,分光光度法,含量测定,
英文关键词:propolis,total flavone,spectrophotometry,content determination,
发表日期：2015-03-16
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