气相色谱-串联质谱法检测水产品中氯霉素残留量

气相色谱-串联质谱法检测水产品中氯霉素残留量
目的  建立检测分析水产品中氯霉素残留量的气相色谱-串联质谱联用(GC-MS/MS)的方法。方法  样品采用乙酸乙酯提取、经过C18固相萃取柱净化, 用硅烷化试剂衍生化, 采用GC-MS/MS分析时, 氯霉素在15 min内流出。结果  对样品添加两种浓度的加标质控, 回收率在80%以上, 相对标准偏差(RSD)在6.2%以内。在0.5~10 mg/L质量浓度范围内呈线性关系, 相关系数大于0.99。结论  该方法可用于氯霉素残留的准确定量分析, 灵敏度、精密度和准确度均能满足渔药残留检测分析要求。

Objective  To establish a determination method of chloramphenicol (CAP) in aquatic products by gas chromatography-tandem mass spectrometry (GC-MS/MS). Methods  The samples were extracted by ethyl acetate, cleaned up by C18 SPE column, and derived with silanization reagent, then the extracts were de-termined by MS/MS detector based on SCAN and MRM mode. Results  CAP was well separated within 15 min. This method showed that the spiked average recoveries above 80%, the RSD ranged within 6.2%, and the calibration of those pesticides were linearity within the range of 0.5～10 mg/L (r2>0.99). Conclusion  The method is accurate and sensitive, it is suitable for analysis of CAP residues in aquatic products.

标题：气相色谱-串联质谱法检测水产品中氯霉素残留量
英文标题：Determination of chloramphenicol residues in aquatic products by gas chro-matography-tandem mass spectrometry

作者：
宋宏娟 新疆维吾尔自治区水环境监测中心

中文关键词:氯霉素,气相色谱-串联质谱法,药物残留,水产品,
英文关键词:chloramphenicol,gas chromatography-tandem mass spectrometry/mass spectrometry,drug residues,aquatic products,

发表日期：2015-04-24

2025-1-29 19:57 上传 气相色谱-串联质谱法检测水产品中氯霉素残留量.pdf

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