响应面法优化油茶籽仁黄酮提取工艺
目的 以总黄酮得率为指标, 用响应面法对乙醇回流提取黄酮的工艺进行优化。方法 通过对提取工艺中的液料比、提取温度、乙醇体积分数、提取时间4个因素进行单因素试验, 筛选出主要的影响因素。以此为基础利用Box-Behnken设计正交试验, 最后结果利用Design-Expert软件进行回归分析并对最佳工艺参数进行验证试验。结果 得到优化提取工艺条件为: 提取温度80 ℃, 乙醇体积分数57%, 液料比53 mL/g, 提取时间2.5 h。在此条件下总黄酮得率的试验值3.91%, 与最大预测值3.94%基本一致。结论 通过响应曲面优化设计提高了油茶籽仁黄酮的得率, 为进一步开发利用油茶籽提供了理论依据。
Objective To optimize extracting technology of total flavonoids from Camellia oleifera seeds with response surface method, taking the extraction rate of total flavonoids as the response indicator. Methods Three factors that affecting extraction rate of total flavonoids were screened out among extraction temperature, ethanol concentration, liquid-solid ratio, and extraction time. The optimum extracting condition and their interaction of the 3 factors were determined through Box-Behnken design and regression analysis using Design-Expert software. Results The optimum extracting condition was: extraction temperature 80 ℃, ethanol concentration 57%, liquid-solid ratio of 53 mL/g， and extraction time 2.5 h. Under this condition the experimental extraction rate of flavonoids was 3.91%, which was consistent with the highest predictive value of 3.94%. Conclusion The optimization of extracting technology for flavonoids from Camellia oleifera seed is feasible and effective, which can provide the theoretical basis for further development and utilization of Camellia oleifera seed.
标题：响应面法优化油茶籽仁黄酮提取工艺
英文标题：Optimizing extraction process of flavonoids from Camellia oleifera seeds by using response surface method
作者：
梁帆 湖南农业大学食品科技学院
郭华 湖南农业大学食品科技学院
周玥 湖南农业大学食品科技学院
中文关键词:油茶籽,黄酮,响应面法,提取工艺,
英文关键词:Camellia oleifera seed,flavonoids,response surface methodology,extraction technology,
发表日期：2015-04-24
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