茶多酚等抗氧化剂对香烟烟雾致损HEK293细胞的保护性研究

茶多酚等抗氧化剂对香烟烟雾致损HEK293细胞的保护性研究
目的  研究茶多酚等天然抗氧化剂对香烟烟雾致损的HEK293细胞的保护效能及JAK2/STAT3 代谢调控机制。方法  香烟过滤嘴中添加茶多酚、白芦藜醇、维生素C、芦丁、槲皮素、绿原酸、花青素、原花青素8种植物提取物后收集烟气, 对 HEK293 细胞进行 MTT 对比研究及运用 Western blot 技术分析其Bcl-2、Bax、STAT3蛋白表达量变化。结果  香烟烟雾可降低HEK293细胞存活率, 呈浓度依赖关系; 上调 HEK293 细胞中 Bcl-2 蛋白相对表达量, 下调 Bax、STAT3 蛋白相对表达量, 且各蛋白变化趋势呈浓度依赖效应; 在过滤嘴中添加茶多酚、白芦藜醇、维生素C、芦丁、槲皮素、绿原酸、花青素、原花青素8种抗氧化剂明显抑制了香烟烟雾导致HEK293细胞死亡, 结果具有显著性差异(P<0.05), 其中以茶多酚效果最佳。结论  8种植物提取物均能对香烟所致HEK293细胞损伤产生一定的保护作用。

Objective  To investigate the metabolic regulation mechanism of tea polyphenols and other natural antioxidants antagonizing cigarette smoke damaging HEK293 cells and its JAK2/STAT3. Methods  Eight kinds of antioxidants, tea polyphenols, resveratrol, vitamin C, rutin, quercetin, chlorogenicacid, antho-cyanins and proanthocyanidins, were added to cigarette filters and the smoke was collected. HEK293 cells were studied by MTT trail data and the protein expression Bcl-2, Bax, and STAT3 were analyzed by Western blot technique. Results  Cigarette smoke could reduce the HEK293 cell survival rates in a dose-dependent manner. With up-regulate Bcl-2 protein relative expression, down-regulate Bax, and STAT3 protein relative expression levels, each protein expression levels were in a dose-dependent effect for HEK293 cells. Comparing to the control, adding 8 kinds of antioxidants to cigarette filters could inhibit the increase of HEK293 cells death rates caused by cigarette smoke obviously. The results had significant differences (P<0.05), and tea polyphenols was the best. Conclusion  Eight kinds of antioxidants can protect HEK293 cell from damage caused by cigarettes smoke.

标题：茶多酚等抗氧化剂对香烟烟雾致损HEK293细胞的保护性研究
英文标题：Researches on tea polyphenols and other antioxidants  protection abilities of HEK293 cells for cigarette smoke damage

作者：
高静 湖南农业大学园艺园林学院茶学系茶学教育部重点实验室
禹利君 湖南农业大学园艺园林学院茶学系茶学教育部重点实验室
周清明 湖南农业大学农学院烟草系
黄建安 湖南农业大学园艺园林学院茶学系茶学教育部重点实验室
刘仲华 湖南农业大学园艺园林学院茶学系茶学教育部重点实验室

中文关键词:香烟烟雾,抗氧化剂,细胞存活率,JAK2/STAT3,
英文关键词:cigarette smoke,antioxidants,cell survival rates,JAK2/STAT3,

发表日期：2015-03-25

2025-1-29 19:56 上传 茶多酚等抗氧化剂对香烟烟雾致损HEK293细胞的保护性研究.pdf

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