分子生物学技术在食源性致病菌检测中的 研究进展
食源性致病菌是导致食源性疾病的重要爆发根源之一。分子生物学检测技术因其敏感性强、特异性高、快速简便、省时省力等优点, 在食源性致病菌的鉴定与检测中扮演着重要角色。本文系统阐述了利用分子生物学技术检测食源性致病菌的方法, 包括多重PCR、实时荧光定量PCR、环介导等温扩增技术、基因芯片、液相芯片和基因探针技术等, 分析了目前国内外学者对于这些技术由单一检测向多重检测的突破, 及实现快速、高通量检测食源性致病菌的应用研究成果, 总结了各种检测技术的优缺点, 旨在为未来更好地发展快速、高通量检测食源性致病菌的技术方法提供参考。
Foodborne diseases are mainly caused by foodborne pathogens. Molecular biological detection technology which possesses the advantages of high sensitivity, high specificity, rapid and easy, and time-saving, and so on plays an important role in identification and detection of food-borne pathogens. This paper elaborated systematically the molecular biology technique adopted in detection of food-borne pathogens, including traditional PCR, multiple PCR, real-time fluorescent quantitative PCR, loop-mediated isothermal amplification technology, gene chips, liquid phase chips, and gene probes, and analyzed the breakthrough of single to multiple detection and research results of rapid and high-throughput detection of food borne pathogens from studies at home and abroad. Finally, the paper summarized the advantages and disadvantages of various detection techniques for the purpose that providing a reference for the better development of rapid, high-throughput detection food-borne pathogens in the future.
标题：分子生物学技术在食源性致病菌检测中的 研究进展
英文标题：Research progress of molecular biology techniques on detecting foodborne pathogens
作者：
唐廷廷 四川农业大学食品学院,重庆三峡职业学院农林科技系
韩国全 四川农业大学食品学院
王利娜 四川省成都市食品药品检验研究院
肖玲 四川农业大学食品学院
王成程 四川农业大学食品学院
文昕 四川农业大学食品学院
中文关键词:分子生物学技术,食源性致病菌,检测方法,食品安全,
英文关键词:molecular biological technology,foodborne pathogens,detection method,food safety,
发表日期：2016-07-29
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