Objective To investigate coliphage MS2 standard material used as quality control material so as to establish the whole process quality control system of RNA virus detection in which the standard material was used as quality control. Methods Standard materials of coliphage MS2 were developed by liquid culture method, and the stability, uniformity and valuing of the standard materials were also determined by double agar plate method. The standard material was applied in real-time fluorescent RT-PCR for detection of norovirus in shellfish to guarantee the quality of whole detection process. Results The standard material had good stability and uniformity and was valued as (1.57±0.0288)×1011 pfu/mL, and the shelf life was determined as 12 months. The extraction efficiencies of virus were between 3.70% and 7.84% in four kinds of shellfish samples, which were according with ISO 15216:2-2013 (virus extraction efficiency should be more than 1%). Conclusion The coliphage MS2 standard material is good at the whole process of quality control in RNA virus detection, which has a promising prospect.
标题：RNA病毒检测质控物质-大肠杆菌噬菌体MS2标准样品的研制和应用
英文标题：Development and application of coliphage MS2 standard material as quality control in RNA virus detection
