高压液相色谱-荧光法检测辣椒干中阿维菌素残留量
目的 建立辣椒干中阿维菌素残留量的高压液相色谱-荧光检测器分析方法。方法 样品采用乙腈提取, 乙腈饱和的正己烷去脂, 石墨化碳固相萃取小柱净化, 以三氟乙酸酐(trifluoroacetic anhtdride, TFAA)和N-甲基咪唑(1-methylimidazole, NMIM)的乙腈溶液衍生化, 用C18柱分离, 在激发波长为365 nm, 发射波长470 nm的条件下高压液相色谱-荧光法检测。结果 方法在10~500 ng/mL的浓度范围内线性关系良好, 相关系数为r=0.999, 检测限为5 μg/kg。3个不同浓度水平上进行了加标回收率在90.3%~103.1%, RSD在2.31%~6.93%。结论 该方法简便灵敏, 准确快速, 可用于辣椒干中阿维菌素残留量的测定。
Objective To establish an HPLC with fluorescence dedection method for determination of avermectin residues in dried chili. Methods Residues are extracted with acetonitrile. The extract was cleaned up with acetonitrile saturated n-hexane and graphitized carbon solid-phase extraction cartridge. Avermectin residues were derived with trifluoroacetic acid and 1-methylimidazole and determined by reversed-phase liquid chromatography with fluorescence detection (excitation: 365 nm and emission: 470 nm). Results Recoveries ranged from 90.3% to 103.1% with RSD of 2.31?6.93% at spiking levels of 5, 10, 20 μg/kg. This method showed a good linearity over the range of 10~500 ng/mL with r=0.999. Conclusion This method is accurate, rapid, and highly sensitive and it is suitable for determination of avermectin residues in dried chili.
标题：高压液相色谱-荧光法检测辣椒干中阿维菌素残留量
英文标题：Determination of avermectin residues in dried chili by HPLC with fluorescence detection
作者：
魏欣欣 河北出入境检验检疫局
窦彩云 河北出入境检验检疫局
段永生 河北出入境检验检疫局
马育松 河北出入境检验检疫局
中文关键词:阿维菌素,高压液相色谱法,辣椒干,农药残留,
英文关键词:avermectin,HPLC-FLD,dried chili,pesticide residues,
发表日期：2013-09-17
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