抗黄曲霉毒素B1独特型纳米抗体的表达及复性

抗黄曲霉毒素B1独特型纳米抗体的表达及复性
目的  快速制备大量具有生物活性的独特型纳米抗体F7。方法  构建pET22b-F7原核表达载体, 转化至大肠杆菌E. coli BL21(DE3)中进行诱导表达, 对诱导温度、诱导剂浓度和诱导时间进行优化。结果  独特型纳米抗体F7表达量有所增加但主要以包涵体形式存在。经过包涵体的溶解、复性获得了具有生物活性的抗AFB1独特型纳米抗体, ELISA证实复性后的蛋白依然存在对鼠源AFB1抗体的结合特性。结论  为后续抗体的性质研究和改造奠定了基础。

Objective  To rapidly produce a large amount of anti-idiotype nanobody against aflatoxin B1 (AFB1). Methods  Prokaryotic expression vector pET22b-F7 were constructed, and then transformed into E.coli BL21(DE3) for expression of anti-idiotype nanobody against aflatoxin B1 (AFB1). The conditions such as temperature, concentration of inducer, and  induction time were optimized. Results  The expression quantity of anti-idiotype nanobody F7 increased, with the main form of inclusion body. Anti-idiotype nanobody against AFB1 with bioactivity were obtained after dissolution and renaturation. ELISA results indicated that the renatured protein had the properties of combination with AFB1 antibody. Conclusion  It provides a reference for further study of properties and subsequent transformation.

标题：抗黄曲霉毒素B1独特型纳米抗体的表达及复性
英文标题：Prokaryotic expression and renaturation of anti-idiotype nanobody against aflatoxin B1

作者：
冯凡 南昌大学食品科学与技术国家重点实验室
许杨 南昌大学食品科学与技术国家重点实验室;中德联合研究院
王丹 南昌大学食品科学与技术国家重点实验室
雷达 南昌大学食品科学与技术国家重点实验室
孙澄浩 南昌大学食品科学与技术国家重点实验室

中文关键词:黄曲霉毒素B1,独特型抗体,纳米抗体,原核表达,复性,
英文关键词:aflatoxin B1,anti-idiotype antibody,nanobody,prokaryotic expression,renaturation,

发表日期：2013-07-16

抗黄曲霉毒素B1独特型纳米抗体的表达及复性.pdf

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