Objective To develop the homologous detection method of metallothionein in vegetables under different heavy metal stress. Methods IgG were collected from Cd-MT immunized rabbit, and metallothionein expression in radish seedlings, pakchoi and leaf-used lettuce were introduced by adding different con-centration of CuSO4, lead acetate, CoCl2 and HgCl2 respectively. Immunoblotting technique was used for homologous detection. Results Metallothionein synthesized in three kinds of vegetables under the stress of Cd-MT and heavy metal were homologous; the concentration of heavy metal influenced the expression of MTs in seeding. Conclusion The IgG collected from Cd-MT immunized rabbit can be used for detection of heavy metal contaminations in agricultural products.
标题：不同重金属胁迫蔬菜产生金属硫蛋白的同源性检测
英文标题：Homologous detection of metallothionein in vegetables under different heavy metal stress
